Structural and functional dynamics of Escherichia coli ribonuclease II : initial studies using a novel fluorescence based system

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Smith, Adam David
University of Lethbridge. Faculty of Arts and Science
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Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2009
Ribonuclease II (RNase II) is a bacterial enzyme responsible for 90% of the exonucleolytic degradation of mRNA in bacteria, and has bacterial homologues known to be involved in virulence. The goal of this project was to examine the structural dynamics of RNase II using fluorescence. Prior to the beginning of this project, little was known regarding the structural composition of RNase II – required information in the study of structural dynamics. Consequently, the structure of RNase II was studied by constructing a series of deletion mutants in order to map the domains. The publication of an atomic resolution structure of RNase II allowed the project to move directly into the study of RNase II structural dynamics as it degrades mRNA. As a step towards this, RNase II was fluorescently labeled, and preliminary binding studies of DNA – a competitive inhibitor – to RNase II using fluorescence were conducted.
xii, 90 leaves : ill. (some col.) ; 29 cm
Ribonucleases -- Research , Escherichia coli -- Research , Messenger RNA -- Research , Dissertations, Academic