Structural and functional dynamics of Escherichia coli ribonuclease II : initial studies using a novel fluorescence based system

Simple item page
dc.contributor.authorSmith, Adam David
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorMosimann, Steven C.
dc.contributor.supervisorWieden, Hans-Joachim
dc.date.accessioned2011-06-02T19:06:15Z
dc.date.available2011-06-02T19:06:15Z
dc.date.issued2009
dc.degree.levelMasters
dc.descriptionxii, 90 leaves : ill. (some col.) ; 29 cmen_US
dc.description.abstractRibonuclease II (RNase II) is a bacterial enzyme responsible for 90% of the exonucleolytic degradation of mRNA in bacteria, and has bacterial homologues known to be involved in virulence. The goal of this project was to examine the structural dynamics of RNase II using fluorescence. Prior to the beginning of this project, little was known regarding the structural composition of RNase II – required information in the study of structural dynamics. Consequently, the structure of RNase II was studied by constructing a series of deletion mutants in order to map the domains. The publication of an atomic resolution structure of RNase II allowed the project to move directly into the study of RNase II structural dynamics as it degrades mRNA. As a step towards this, RNase II was fluorescently labeled, and preliminary binding studies of DNA – a competitive inhibitor – to RNase II using fluorescence were conducted.en_US
dc.identifier.urihttps://hdl.handle.net/10133/1289
dc.language.isoen_USen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2009en_US
dc.publisher.departmentDepartment of Chemistry and Biologyen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectRibonucleases -- Researchen_US
dc.subjectEscherichia coli -- Researchen_US
dc.subjectMessenger RNA -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.titleStructural and functional dynamics of Escherichia coli ribonuclease II : initial studies using a novel fluorescence based systemen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
SMITH_ADAM_DAVID_MSC_2009.pdf
Size:
4.46 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.63 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Arts and Science, Faculty of
University of Lethbridge Theses