Characterization of Mannheimia haemolytica-specific bacteriophages

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Hsu, Yu-Hung
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Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2011
Mannheimia haemolytica is the principal bacterial agent associated with bovine respiratory disease (BRD). It has a significant economic impact on the beef feedlot industry. The current methods for BRD prevention and treatment have various problems and limitations, especially with reports of increased antimicrobial resistance in M. haemolytica. Bacteriophage therapy presents a novel method to mitigate M. haemolytica. This study aimed to isolate strictly lytic M. haemolytica-specific bacteriophages from bovine nasopharyngeal swabs and feedlot trough water. This was accompanied by an extensive characterization of temperate bacteriophages induced from representative strains of a M. haemolytica collection. Phage morphology, host specificity, genomic diversity, and comparative genomics were determined. Even though temperate bacteriophages are not ideal candidates for phage therapy, they can be engineered or modified to serve this function. Genome sequences of selected temperate bacteriophages also provide a foundation for future studies on the biology of these microorganisms.
viii, 107 leaves : ill. ; 29 cm
Bacteriophages -- Genetics , Bacteriophages -- Research , Bacteriophages -- Therapeutic use , Bovine pneumonic pasteurellosis -- Treatment -- Research , Bovine pneumonic pasteurellosis -- Prevention -- Research , Communicable diseases in animals -- Treatment -- Research , Dissertations, Academic