Zinc-finger protein CNBP alters the 3-D structure of IncRNA Braveheart in solution

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dc.contributor.authorKim, Doo N.
dc.contributor.authorThiel, Bernhard C.
dc.contributor.authorMrozowich, Tyler
dc.contributor.authorHennelly, Scott P.
dc.contributor.authorHofacker, Ivo L.
dc.contributor.authorPatel, Trushar R.
dc.contributor.authorSanbonmatsu, Karissa Y.
dc.date.accessioned2024-06-19T21:44:22Z
dc.date.available2024-06-19T21:44:22Z
dc.date.issued2020
dc.descriptionOpen access article. Creative Commons Attribution 4.0 International license (CC BY 4.0) applies
dc.description.abstractLong non-coding RNAs (lncRNAs) constitute a significant fraction of the transcriptome, playing important roles in development and disease. However, our understanding of structure-function relationships for this emerging class of RNAs has been limited to secondary structures. Here, we report the 3-D atomistic structural study of epigenetic lncRNA, Braveheart (Bvht), and its complex with CNBP (Cellular Nucleic acid Binding Protein). Using small angle X-ray scattering (SAXS), we elucidate the ensemble of Bvht RNA conformations in solution, revealing that Bvht lncRNA has a well-defined, albeit flexible 3-D structure that is remodeled upon CNBP binding. Our study suggests that CNBP binding requires multiple domains of Bvht and the RHT/AGIL RNA motif. We show that RHT/AGIL, previously shown to interact with CNBP, contains a highly flexible loop surrounded by more ordered helices. As one of the largest RNA-only 3-D studies, the work lays the foundation for future structural studies of lncRNA-protein complexes.
dc.identifier.citationKim, D. N., Thiel, B. C., Mrozowich, T., Hennelly, S. P., Hofacker, I. L., Patel, T. R., & Sanbonmatsu, K. Y. (2020). Zinc-finger protein CNBP alters the 3-D structure of IncRNA Braveheart in solution. Nature Communications, 11, Article 148. https://doi.org/10.1038/s41467-019-13942-4
dc.identifier.urihttps://hdl.handle.net/10133/6790
dc.language.isoen
dc.publisherNature Portfolio
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.publisher.institutionLos Alamos National Laboratory
dc.publisher.institutionUniversity of Vienna
dc.publisher.institutionUniversity of Lethbridge
dc.publisher.institutionNew Mexico Consortium
dc.publisher.institutionUniversity of Calgary
dc.publisher.institutionUniversity of Alberta
dc.subjectBiophysical chemistry
dc.subjectBiophysics
dc.subjectComputational biophysics
dc.subjectMolecular biophysics
dc.subjectRNA
dc.subjectIncRNA
dc.subject3-D studies
dc.subject.lcshProtein complexes
dc.titleZinc-finger protein CNBP alters the 3-D structure of IncRNA Braveheart in solution
dc.typeArticle
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