Modulation of the immune system in the mammalian intestine as an alternate explanation for the action of antimicrobial growth promoters / Estela Costa
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Date
2010
Authors
Costa, Estela
University of Lethbridge. Faculty of Arts and Science
Journal Title
Journal ISSN
Volume Title
Publisher
Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2010
Abstract
The novel hypothesis that antimicrobial growth promoters (AGP) function by
modulating the mammalian immune system was tested. Sampling methods to
characterize the mucosa-associated microbiota of the murine intestine by terminal
restriction fragment length polymorphism (T-RFLP) analysis indicated that direct plug
extraction was superior to wash methods. Using T-RFLP analysis, non-therapeutic
administration of chlortetracycline (CTC) and sulfamethazine to beef cattle did not affect
the composition of bacterial communities associated with intestinal mucosa and in
digesta, with exception of those associated with mucosa of the proximal jejunum.
Similarly, oral administration of non-therapeutic concentrations of CTC did not affect the
mucosa-associated microbiota of the murine intestine. Oral administration of nontherapeutic
concentrations of CTC prevented weight loss, reduced pathologic changes,
modulated transcription levels of inflammatory cytokines in C. rodentium-infected mice,
and did not consistently affect the colonic microbiota. These findings support the
hypothesis that AGP primarily function by modulating the intestinal immune system.
Description
xiv, 160 leaves ; 29 cm
Keywords
Antibiotics in animal nutrition , Antibiotic residues -- Health aspects , Drug resistance in microorganisms , Antibiotics in veterinary medicine , Food of animal origin -- Contamination , Veterinary drug residues , Dissertations, Academic