The arabidopsis gene Grassy, is required for auxin transport and patterning of leaf vein, shoot and root
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Date
2008
Authors
Pahari, Shankar
University of Lethbridge. Faculty of Arts and Science
Journal Title
Journal ISSN
Volume Title
Publisher
Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2008
Abstract
Auxin controls a range of growth related characteristics by a mechanism
dependent upon polar auxin transport. We have identified a leaf vein patterning mutant
that shows a simple first leaf vein pattern. The veins are often non-meeting and form
somewhat parallel to one another. The leaves are narrow and pointed so that the overall
leaf phenotype is reminiscent of grass leaves; hence the mutant name grassy (gsy). A
range of shoot and root characteristics are also altered in gsy plants. Compared to wild
type, gsy plants have shorter primary roots with reduced numbers of lateral roots and
increased numbers of longer root hairs. Upon gravitropic stimulation, the root tip bends
slightly away from the normal vector. As well, gsy plants produce an inflorescence with
altered internode elongation and branching pattern. The intensity of the auxin responsive
reporter gene DR5::GUS is unchanged in both roots and developing leaves of gsy,
however, it shows subtle differences to the wild type DR5:GUS expression pattern.
Finally, gsy leaf and root phenotypes are more sensitive to low doses of the auxin efflux
inhibitor NPA and external auxin 2, 4-D. We suggest that this overall pattern is
consistent with defects in auxin transport.
Description
xii, 76 leaves : ill. ; 29 cm.
Keywords
Dissertations, Academic , Plants -- Effect of auxin on , Auxin , Arabidopsis