Kinetic analyses on two translational GTPases : LepA and EF-Tu

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De Laurentiis, Evelina I.
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Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
Protein synthesis is an essential process for all living organisms and is an effective major target for current antibiotics. Elongation factor Tu (EF-Tu) is a highly conserved and essential protein that functions during protein synthesis. EF-Ts interacts with EF-Tu to help maintain a functionally active state of EF-Tu required for cell growth. Although EF-Ts is essential for Escherichia coli, its sequence is poorly conserved. LepA is a highly conserved protein within bacteria and has a similar structure to EF-Tu. In spite of this, LepA has been shown to be non-essential under ideal conditions and the function of LepA still remains elusive. An analysis on the structurally unique aspects of LepA, EF-Tu and EF-Ts was performed here in an effort to gain an understanding on the functions of these proteins. This knowledge, in combination with their unique structural components will provide important tools in developing new and effective antibiotics.
xiii, 177 leaves : col. ill. ; 29 cm
Guanosine triphosphatase , Signal peptidases , Genetic translation , Proteins -- Synthesis