The down-regulation of Ku70, DNA-PKcs, and Parp-1 in mammalian cell lines

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Wickersham, Stephanie
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Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2012
DNA double strand breaks (DSBs) are primarily repaired in eukaryotic cells by two different mechanisms – non-homologous end joining (NHEJ) or homologous recombination (HR). In mammalian somatic cells the balance between the two highly favours NHEJ. Gene targeting is a technique that exploits HR repair to alter a defined gene locus. While it holds potential to be implemented as a treatment option for several diseases, the outlook for using it in a clinical setting has been obstructed by a low gene targeting efficiency. This has been coupled to the low frequency of HR in mammalian cells. With the intention of shifting the repair balance, antibodies against DSB repair proteins will be introduced into mammalian cells. It is predicted that by targeting key repair proteins with antibodies, a compensatory increase in the frequency of HR can be fostered, ultimately resulting in improved gene targeting.
xv, 168 leaves : ill. ; 29 cm
DNA damage -- Research , DNA repair -- Research , Gene targeting -- Research , Protein kinases , NAD-ADP-ribosyltransferase