The down-regulation of Ku70, DNA-PKcs, and Parp-1 in mammalian cell lines

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dc.contributor.authorWickersham, Stephanie
dc.contributor.supervisorKovalchuk, Igor
dc.date.accessioned2014-04-16T23:35:45Z
dc.date.available2014-04-16T23:35:45Z
dc.date.issued2012
dc.degree.levelMasters
dc.descriptionxv, 168 leaves : ill. ; 29 cmen_US
dc.description.abstractDNA double strand breaks (DSBs) are primarily repaired in eukaryotic cells by two different mechanisms – non-homologous end joining (NHEJ) or homologous recombination (HR). In mammalian somatic cells the balance between the two highly favours NHEJ. Gene targeting is a technique that exploits HR repair to alter a defined gene locus. While it holds potential to be implemented as a treatment option for several diseases, the outlook for using it in a clinical setting has been obstructed by a low gene targeting efficiency. This has been coupled to the low frequency of HR in mammalian cells. With the intention of shifting the repair balance, antibodies against DSB repair proteins will be introduced into mammalian cells. It is predicted that by targeting key repair proteins with antibodies, a compensatory increase in the frequency of HR can be fostered, ultimately resulting in improved gene targeting.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3401
dc.language.isoen_CAen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2012en_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.subjectDNA damage -- Researchen_US
dc.subjectDNA repair -- Researchen_US
dc.subjectGene targeting -- Researchen_US
dc.subjectProtein kinasesen_US
dc.subjectNAD-ADP-ribosyltransferaseen_US
dc.titleThe down-regulation of Ku70, DNA-PKcs, and Parp-1 in mammalian cell linesen_US
dc.typeThesisen_US
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