Control of substrate utilization by O-islands and S-loops in Escherichia coli O157:H7

Simple item page
dc.contributor.authorPaquette, Sarah-Jo
dc.contributor.supervisorThomas, James E.
dc.contributor.supervisorGannon, Victor P. J.
dc.date.accessioned2012-07-20T00:25:32Z
dc.date.available2012-07-20T00:25:32Z
dc.date.issued2011
dc.degree.levelMasters
dc.descriptionxx, 208 leaves : ill. (some col.) ; 29 cmen_US
dc.description.abstractEscherichia coli O157:H7 is an enteric pathogen that can cause severe gastrointestinal disease, sometimes leading to hospitalization and death. These bacteria have a variety of virulence factors that can be encoded for on pathogenicity islands (PAIs). The goal of this study was to characterize specific E. coli O157:H7 PAI deletion mutants using three methods: Phentotype Microarrays (PM), growth curves and survival curves were used to elucidate possible roles for the PAIs. Results from the PM study suggest that PAIs have a role in carbon substrate utilization; i.e., four of the O-island (OI) deletion mutants (OI-87, 98, 102 and 172) and an S-Loop (SL-72) deletion mutant exhibited differences in substrate utilization (gains and losses in utilization) compared to parental O157:H7 strains EDL933 (OI) and Sakai (SL), respectively. All of the mutants with the exception of the OI-135 mutant exhibited differences in level of substrate utilization for substrates shown to have important roles in the bacterium. Cell growth results showed that three OI deletion mutants (OI-55, 87 and 102) and the SL (SL-72) mutant exhibited a difference in rate of growth compared to the parental strains. Cell viability results showed that seven of the OI deletion mutants (OI-51, 55, 98, 108, 135, 172 and 176) exhibited different rates of decline in cell number when transferred to sterile water compared to the parental strain. The results show that removal of PAIs from E. coli O157:H7 can affect carbon utilization, growth and survival demonstrating the importance of PAIs in the ecology of these bacteria.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3104
dc.language.isoen_USen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2011en_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectEscherichia coli O157:H7 -- Researchen_US
dc.subjectEscherichia coli O157:H7 -- Genetics -- Experimentsen_US
dc.subjectMutagenesisen_US
dc.subjectDNA microarraysen_US
dc.subjectGene expressionen_US
dc.subjectMicrobial mutationen_US
dc.subjectDissertations, Academicen_US
dc.titleControl of substrate utilization by O-islands and S-loops in Escherichia coli O157:H7en_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
PAQUETTE_SARAH_JO_MSC_2011.pdf
Size:
973.62 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.63 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Arts and Science, Faculty of
University of Lethbridge Theses