Tissue-specific gene expression and promoter characterization in triticale

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Penniket, Carolyn Renee
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Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2013
Triticale (x Triticosecale Whitm.) is a cereal with favorable agronomic traits for a Canadian bioproduction platform crop. Appropriate tissue sampling times were determined and gene expression profiles were evaluated in five triticale seed tissues and eleven vegetative tissues using the Affymetrix Wheat GeneChip®. Genes that were expressed, not expressed, tissue-specific, tissue-enriched and developmentally regulated were identified. The percentage of probe sets on the wheat GeneChip with gene ontology annotations was improved from less than 3% to over 76% using homologous sequence identification and annotation transfer. This information was used to determine functions and processes over-represented within the identified gene lists and provide biological meaning to the results. Expression of candidate genes was further evaluated using qRT-PCR, RNA in situ hybridization and promoter characterization. This study has provided a comprehensive triticale gene expression atlas; knowledge regarding triticale development, gene function, expression and regulation; and tools enabling further triticale research and development.
xxiii, 425 leaves : col. ill. ; 29 cm
Triticale -- Genetics -- Research , Triticale -- Biotechnology , Gene expression -- Research , Promoters (Genetics) -- Research , Plant biotechnology -- Research , Dissertations, Academic