Tissue-specific gene expression and promoter characterization in triticale

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dc.contributor.authorPenniket, Carolyn Renee
dc.contributor.supervisorLaroche, Andre
dc.contributor.supervisorSelinger, L. Brent
dc.date.accessioned2014-02-28T22:42:57Z
dc.date.available2014-02-28T22:42:57Z
dc.date.issued2013
dc.degree.levelPhD
dc.descriptionxxiii, 425 leaves : col. ill. ; 29 cmen_US
dc.description.abstractTriticale (x Triticosecale Whitm.) is a cereal with favorable agronomic traits for a Canadian bioproduction platform crop. Appropriate tissue sampling times were determined and gene expression profiles were evaluated in five triticale seed tissues and eleven vegetative tissues using the Affymetrix Wheat GeneChip®. Genes that were expressed, not expressed, tissue-specific, tissue-enriched and developmentally regulated were identified. The percentage of probe sets on the wheat GeneChip with gene ontology annotations was improved from less than 3% to over 76% using homologous sequence identification and annotation transfer. This information was used to determine functions and processes over-represented within the identified gene lists and provide biological meaning to the results. Expression of candidate genes was further evaluated using qRT-PCR, RNA in situ hybridization and promoter characterization. This study has provided a comprehensive triticale gene expression atlas; knowledge regarding triticale development, gene function, expression and regulation; and tools enabling further triticale research and development.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3373
dc.language.isoen_CAen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2013en_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.subjectTriticale -- Genetics -- Researchen_US
dc.subjectTriticale -- Biotechnologyen_US
dc.subjectGene expression -- Researchen_US
dc.subjectPromoters (Genetics) -- Researchen_US
dc.subjectPlant biotechnology -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.titleTissue-specific gene expression and promoter characterization in triticaleen_US
dc.typeThesisen_US
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