Applications of manganese-enhanced magnetic resonance imaging in neuroscience
McCreary, J. Keiko
Lethbridge, Alta. : University of Lethbridge, Dept. of Neuroscience, c2012
Manganese-Enhanced Magnetic Resonance Imaging (MEMRI) has proven itself to be a beneficial technique in the field of Neuroscience. This thesis applies MEMRI to studies in neuroscience by first establishing the limitations concerning the use of MEMRI in live rats. Experiment 1 used an osmotic pump for manganese (Mn) delivery to the lateral ventricles for acquisition of anatomical images using MEMRI. From my knowledge, this was the first method demonstrating slow infusion of Mn to the lateral ventricles. In Experiment 2, MEMRI was used for volumetric analysis the whole brain and hippocampus of prenatally stressed rats. To my knowledge, this study was the first to investigate the effect of generational prenatal stress on the structure of a rat’s brain using MEMRI and histology. Additionally, Experiment 2 investigated the use of a subcutaneous osmotic pump to deliver Mn for MEMRI. A summary on the use of MEMRI in Neuroscience concludes this thesis, with a discussion on the methods used and related technical considerations.
xi, 84 leaves ; 29 cm
Brain -- Magnetic resonance imaging , Rats -- Effect of stress on , Magnetic resonance imaging , Neurosciences , Dissertations, Academic