Functional role of the conserved amino acids Cysteine 81, Arginine 279, Glycine 280 and Arginine 283 in elongation factor Tu from Escherichia coli

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dc.contributor.authorMo, Fan
dc.contributor.supervisorWieden, Hans-Joachim
dc.date.accessioned2012-07-30T22:20:15Z
dc.date.available2012-07-30T22:20:15Z
dc.date.issued2011
dc.degree.levelMasters
dc.descriptionx, 85 leaves : ill. (some col.) ; 29 cmen_US
dc.description.abstractDuring protein synthesis, elongation factor Tu (EF-Tu) delivers aminoacyl-tRNA (aa-tRNA) to the A-site of mRNA-programmed ribosomes in a GTP-dependent manner. To enable future studies on the functional and structural requirement of EF-Tu’s function, a Cysteine-free variant of EF-Tu was constructed suitable for subsequent labelling of the protein and use in kinetic studies. Here, the kinetic properties of three Cysteine-less EF-Tu variants are reported, demonstrating that only the variant with the Alanine substitution in position 81 retains wild-type activity with respect to the interaction with guanine nucleotides, aa-tRNA and the ribosome. To explore a possible tRNA independent pathway for the GTPase activation signal, three residues in domain II of EF-Tu (Arginine 279, Glycine 280, Arginine 283) were mutated; the activity of EF-Tu variants were analyzed. Results suggest that these residues are indeed required for efficient ribosome-dependent stimulation of the GTPase activity of EF-Tu.en_US
dc.identifier.urihttps://hdl.handle.net/10133/3107
dc.language.isoen_USen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry, c2011en_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectAminoacyl-tRNAen_US
dc.subjectGuanosine triphosphataseen_US
dc.subjectProteins -- Synthesisen_US
dc.subjectBinding sites (Biochemistry)en_US
dc.subjectGenetic translationen_US
dc.subjectEscherichia colien_US
dc.subjectDissertations, Academicen_US
dc.titleFunctional role of the conserved amino acids Cysteine 81, Arginine 279, Glycine 280 and Arginine 283 in elongation factor Tu from Escherichia colien_US
dc.typeThesisen_US
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