Generating plant lines with markers designed to assess cell polarity defects at the molecular level
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Date
2021
Authors
Pageni, Utsab
University of Lethbridge. Faculty of Arts and Science
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Publisher
Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences
Abstract
Cellular polarity is a fundamental aspect of all multicellular organisms that enables certain cell types to carry out their functions. Therefore, cell polarity formation is an important basis for understanding the molecular linkage between morphology and cellular functions. In Arabidopsis, the position of root hairs is mediated by intercellular flow of the phytohormone auxin, for which directional signaling is determined by the polar subcellular localization of PIN-FORMED (PIN) transport proteins. My research primarily focuses on establishing plant lines that will later allow correlation of changes in proteins distribution with changes in cell polarity and plant morphology. Mutations in genes such as FORKED1, SCARFACE, PINOID and RON3 result in defective localization of PIN proteins in various tissues, by affecting PIN phosphorylation status (PID and RON3) or endomembrane dynamics (FORKED1 and SCARFACE). This study will introduce fluorophore labeled fusion proteins into FORKED1, SCARFACE, PINOID and RON3 mutants.
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Keywords
auxin , PIN , cell polarity , protein localization , Auxin -- Research , Auxin -- Molecular aspects -- Research , Polarity (Biology) -- Research , Root hairs , Plant regulators -- Research , Arabidopsis -- Roots -- Development -- Research , Arabidopsis -- Roots -- Formation -- Research , Arabidopsis -- Roots -- Morphology -- Research , Roots (Botany) -- Morphology -- Research , Dissertations, Academic , Carrier proteins