Generating plant lines with markers designed to assess cell polarity defects at the molecular level

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dc.contributor.authorPageni, Utsab
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorSchultz, Elizabeth
dc.date.accessioned2021-10-18T20:03:01Z
dc.date.available2021-10-18T20:03:01Z
dc.date.issued2021
dc.degree.levelMastersen_US
dc.description.abstractCellular polarity is a fundamental aspect of all multicellular organisms that enables certain cell types to carry out their functions. Therefore, cell polarity formation is an important basis for understanding the molecular linkage between morphology and cellular functions. In Arabidopsis, the position of root hairs is mediated by intercellular flow of the phytohormone auxin, for which directional signaling is determined by the polar subcellular localization of PIN-FORMED (PIN) transport proteins. My research primarily focuses on establishing plant lines that will later allow correlation of changes in proteins distribution with changes in cell polarity and plant morphology. Mutations in genes such as FORKED1, SCARFACE, PINOID and RON3 result in defective localization of PIN proteins in various tissues, by affecting PIN phosphorylation status (PID and RON3) or endomembrane dynamics (FORKED1 and SCARFACE). This study will introduce fluorophore labeled fusion proteins into FORKED1, SCARFACE, PINOID and RON3 mutants.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6064
dc.language.isoen_USen_US
dc.proquest.subject0379en_US
dc.proquest.subject0307en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectauxinen_US
dc.subjectPINen_US
dc.subjectcell polarityen_US
dc.subjectprotein localizationen_US
dc.subjectAuxin -- Researchen_US
dc.subjectAuxin -- Molecular aspects -- Researchen_US
dc.subjectPolarity (Biology) -- Researchen_US
dc.subjectRoot hairsen_US
dc.subjectPlant regulators -- Researchen_US
dc.subjectArabidopsis -- Roots -- Development -- Researchen_US
dc.subjectArabidopsis -- Roots -- Formation -- Researchen_US
dc.subjectArabidopsis -- Roots -- Morphology -- Researchen_US
dc.subjectRoots (Botany) -- Morphology -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.subjectCarrier proteinsen_US
dc.titleGenerating plant lines with markers designed to assess cell polarity defects at the molecular levelen_US
dc.typeThesisen_US
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