Identification and comparison of non-coding RNAs and ribonucleoprotein complexes in several diverse protist species

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dc.contributor.authorMcWatters, David Casey
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorRussell, Anthony G.
dc.date.accessioned2022-07-04T16:45:06Z
dc.date.available2022-07-04T16:45:06Z
dc.date.issued2022
dc.degree.levelPh.Den_US
dc.description.abstractMany different classes of non-coding (nc)RNAs are found in species throughout the tree of life, each of which performs important cellular functions. The objective of my thesis was to identify and characterize ncRNAs and their associated protein complexes, with particular focus on small nucleolar RNAs (snoRNA), in unicellular eukaryotes whose genomes have undergone significant reduction or expansion. To do this I utilized the unicellular eukaryotes Giardia lamblia, Giardia muris and Euglena gracilis. A diRNP containing RNase P and snoRNA domains that targets tRNAMet 2ʹ-O-methylation and the U3 snoRNA were discovered and characterized in two Giardia species. Unique binding/assembly properties were determined for C/D snoRNP proteins in G. lamblia. A large collection of E. gracilis snoRNAs were discovered leading to a better understanding Ψ-guide snoRNA structure and evolution. These findings highlight the diversity of ncRNA features that exist in less well studied eukaryotic species, and their unique functions.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6258
dc.language.isoen_USen_US
dc.proquest.subject0307en_US
dc.proquest.subject0487en_US
dc.proquest.subject0715en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectsnoRNAen_US
dc.subjectsnRNAen_US
dc.subjectGiardia lambliaen_US
dc.subjectEuglena gracilisen_US
dc.subjecttelomeraseen_US
dc.subjectevolutionen_US
dc.subjectNon-coding RNAen_US
dc.subjectEukaryotic cells--Geneticsen_US
dc.subjectGiardia lamblia--Researchen_US
dc.subjectEuglena gracilis--Researchen_US
dc.subjectTelormeraseen_US
dc.subjectEvolution (Biology)en_US
dc.subjectNucleoproteinsen_US
dc.subjectProtistaen_US
dc.subjectRNA-protein interactionsen_US
dc.subjectDissertations, Academicen_US
dc.titleIdentification and comparison of non-coding RNAs and ribonucleoprotein complexes in several diverse protist speciesen_US
dc.typeThesisen_US
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