Members of the Arabidopsis thaliana FL family act with ARF machinery to localize PIN1 during auxin related developmental processes
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Date
2018
Authors
Yu, Houlin
University of Lethbridge. Faculty of Arts and Science
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Lethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciences
Abstract
PINFORMED (PIN) proteins are phytohormone auxin efflux carriers. By polar distribution and dynamic re-localization via the endomembrane vesicle trafficking system, PIN proteins control many auxin-dependent developmental processes. Vesicle trafficking is controlled by protein machineries including small G protein ADP ribosylation factors (ARFs) and their regulators ARF-GTPase activating proteins (ARF-GAPs) and ARF-guanine nucleotide exchange factors (ARF-GEFs). FORKED1 (FKD1) is part of the nine member Arabidopsis FKD1-LIKE (FL) gene family. My results show that FKD1 and SCARFACE (SFC/FKD2, an ARF-GAP) co-localize with BIGs (ARF-GEFs) and they all co-localize with PIN1 in vesicles. Mutant analysis supports the idea that FKD1, SFC and BIGs are acting in the same secretory pathway, and together with ARFA group, these proteins control PIN1 localization and therefore the auxin transport in leaves, hypocotyls and roots. My results also show that FL3 is acting in a different manner from FKD1, likely in the endocytic pathway.
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Keywords
Arabidopsis thaliana -- Genetics -- Research , Plant hormones -- Research , Auxin -- Research , Membrane proteins -- Research , G proteins , Guanosine triphosphatase , GTPase activating protein , endomembrane vesicle trafficking , FKD1-LIKE family , PIN1 localization , SCARFACE , secretory pathway