Members of the Arabidopsis thaliana FL family act with ARF machinery to localize PIN1 during auxin related developmental processes

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dc.contributor.authorYu, Houlin
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorSchultz, Elizabeth
dc.date.accessioned2018-11-06T19:01:25Z
dc.date.available2018-11-06T19:01:25Z
dc.date.issued2018
dc.degree.levelMastersen_US
dc.description.abstractPINFORMED (PIN) proteins are phytohormone auxin efflux carriers. By polar distribution and dynamic re-localization via the endomembrane vesicle trafficking system, PIN proteins control many auxin-dependent developmental processes. Vesicle trafficking is controlled by protein machineries including small G protein ADP ribosylation factors (ARFs) and their regulators ARF-GTPase activating proteins (ARF-GAPs) and ARF-guanine nucleotide exchange factors (ARF-GEFs). FORKED1 (FKD1) is part of the nine member Arabidopsis FKD1-LIKE (FL) gene family. My results show that FKD1 and SCARFACE (SFC/FKD2, an ARF-GAP) co-localize with BIGs (ARF-GEFs) and they all co-localize with PIN1 in vesicles. Mutant analysis supports the idea that FKD1, SFC and BIGs are acting in the same secretory pathway, and together with ARFA group, these proteins control PIN1 localization and therefore the auxin transport in leaves, hypocotyls and roots. My results also show that FL3 is acting in a different manner from FKD1, likely in the endocytic pathway.en_US
dc.description.sponsorshipNSERC Discovery Granten_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5238
dc.language.isoen_USen_US
dc.proquest.subject0379en_US
dc.proquest.subject0369en_US
dc.proquest.subject0758en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectArabidopsis thaliana -- Genetics -- Researchen_US
dc.subjectPlant hormones -- Researchen_US
dc.subjectAuxin -- Researchen_US
dc.subjectMembrane proteins -- Researchen_US
dc.subjectG proteinsen_US
dc.subjectGuanosine triphosphataseen_US
dc.subjectGTPase activating proteinen_US
dc.subjectendomembrane vesicle traffickingen_US
dc.subjectFKD1-LIKE familyen_US
dc.subjectPIN1 localizationen_US
dc.subjectSCARFACEen_US
dc.subjectsecretory pathwayen_US
dc.titleMembers of the Arabidopsis thaliana FL family act with ARF machinery to localize PIN1 during auxin related developmental processesen_US
dc.typeThesisen_US
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