Biophysical characterisation studies of hantaviral and human long noncoding RNAs

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D'Souza, Michael H.
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Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
Emerging zoonotic viruses are a global threat that can cause devastating outbreaks and indelible economic fallout to human systems. North American Hantavirus is a NIAID Category A Priority Pathogen, that regularly involves their Noncoding Terminal Regions to instigate viral replication and translation. Long Noncoding RNAs are integral regulatory molecules involved in a myriad of biological processes. This thesis seeks to identify human RNA-Binding Proteins involved in Hantaviral lifecycles, and to biophysically characterise human Long Noncoding RNAs that mediate cancer progression. Pull-Down Assays identified several RNA stabilising and salient viral lifecycle promoting proteins that putatively interact with Hantaviral Noncoding Terminal Regions. Small Angle X-ray Scattering and coarse-grained SimRNA computational modelling methods generated combined low/high-resolution three-dimensional structures of Long Intergenic Noncoding RNA-p21 Alu Inverted Repeats. Furthermore, we have employed orthogonal biophysical approaches involving Analytical Ultracentrifugation and Multiangle/Dynamic Light Scattering techniques to verify target in vitro transcribed RNA for accuracy.
RNA , Biochemistry , Hantavirus , LincRNA-p21 AluSx1 , RNA-Binding Proteins , Small Angle X-ray Scattering , Dissertations, Academic , Hantaviruses