Biophysical characterisation studies of hantaviral and human long noncoding RNAs

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dc.contributor.authorD'Souza, Michael H.
dc.contributor.supervisorPatel, Trushar R.
dc.date.accessioned2022-01-11T20:04:11Z
dc.date.available2022-01-11T20:04:11Z
dc.date.issued2022
dc.degree.levelMastersen_US
dc.description.abstractEmerging zoonotic viruses are a global threat that can cause devastating outbreaks and indelible economic fallout to human systems. North American Hantavirus is a NIAID Category A Priority Pathogen, that regularly involves their Noncoding Terminal Regions to instigate viral replication and translation. Long Noncoding RNAs are integral regulatory molecules involved in a myriad of biological processes. This thesis seeks to identify human RNA-Binding Proteins involved in Hantaviral lifecycles, and to biophysically characterise human Long Noncoding RNAs that mediate cancer progression. Pull-Down Assays identified several RNA stabilising and salient viral lifecycle promoting proteins that putatively interact with Hantaviral Noncoding Terminal Regions. Small Angle X-ray Scattering and coarse-grained SimRNA computational modelling methods generated combined low/high-resolution three-dimensional structures of Long Intergenic Noncoding RNA-p21 Alu Inverted Repeats. Furthermore, we have employed orthogonal biophysical approaches involving Analytical Ultracentrifugation and Multiangle/Dynamic Light Scattering techniques to verify target in vitro transcribed RNA for accuracy.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6127
dc.language.isoen_USen_US
dc.proquestyesNoen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentBiochemistry and Chemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectRNAen_US
dc.subjectBiochemistryen_US
dc.subjectHantavirusen_US
dc.subjectLincRNA-p21 AluSx1en_US
dc.subjectRNA-Binding Proteinsen_US
dc.subjectSmall Angle X-ray Scatteringen_US
dc.subjectDissertations, Academicen_US
dc.subjectHantavirusesen_US
dc.titleBiophysical characterisation studies of hantaviral and human long noncoding RNAsen_US
dc.typeThesisen_US
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