Biophysical characterisation studies of hantaviral and human long noncoding RNAs
dc.contributor.author | D'Souza, Michael H. | |
dc.contributor.supervisor | Patel, Trushar R. | |
dc.date.accessioned | 2022-01-11T20:04:11Z | |
dc.date.available | 2022-01-11T20:04:11Z | |
dc.date.issued | 2022 | |
dc.degree.level | Masters | en_US |
dc.description.abstract | Emerging zoonotic viruses are a global threat that can cause devastating outbreaks and indelible economic fallout to human systems. North American Hantavirus is a NIAID Category A Priority Pathogen, that regularly involves their Noncoding Terminal Regions to instigate viral replication and translation. Long Noncoding RNAs are integral regulatory molecules involved in a myriad of biological processes. This thesis seeks to identify human RNA-Binding Proteins involved in Hantaviral lifecycles, and to biophysically characterise human Long Noncoding RNAs that mediate cancer progression. Pull-Down Assays identified several RNA stabilising and salient viral lifecycle promoting proteins that putatively interact with Hantaviral Noncoding Terminal Regions. Small Angle X-ray Scattering and coarse-grained SimRNA computational modelling methods generated combined low/high-resolution three-dimensional structures of Long Intergenic Noncoding RNA-p21 Alu Inverted Repeats. Furthermore, we have employed orthogonal biophysical approaches involving Analytical Ultracentrifugation and Multiangle/Dynamic Light Scattering techniques to verify target in vitro transcribed RNA for accuracy. | en_US |
dc.identifier.uri | https://hdl.handle.net/10133/6127 | |
dc.language.iso | en_US | en_US |
dc.proquestyes | No | en_US |
dc.publisher | Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry | en_US |
dc.publisher.department | Biochemistry and Chemistry | en_US |
dc.publisher.department | Department of Chemistry and Biochemistry | en_US |
dc.publisher.faculty | Arts and Science | en_US |
dc.relation.ispartofseries | Thesis (University of Lethbridge. Faculty of Arts and Science) | en_US |
dc.subject | RNA | en_US |
dc.subject | Biochemistry | en_US |
dc.subject | Hantavirus | en_US |
dc.subject | LincRNA-p21 AluSx1 | en_US |
dc.subject | RNA-Binding Proteins | en_US |
dc.subject | Small Angle X-ray Scattering | en_US |
dc.subject | Dissertations, Academic | en_US |
dc.subject | Hantaviruses | en_US |
dc.title | Biophysical characterisation studies of hantaviral and human long noncoding RNAs | en_US |
dc.type | Thesis | en_US |