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dc.contributor.supervisor Abbott, D. Wade
dc.contributor.supervisor Yevtushenko, Dmytro
dc.contributor.author Klassen, Leeann
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2020-01-22T22:14:47Z
dc.date.available 2020-01-22T22:14:47Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/10133/5671
dc.description.abstract The rumen of beef cattle houses a diverse community of microorganisms that impact feed digestion, nutrient accessibility, host health, and waste production. Fibrolytic enzymes, probiotics, and prebiotics are promising candidates for next-generation feed additives, with the goal of inducing beneficial changes to the rumen microbiome. Current methods to investigate the mechanistic interactions between bacteria and feed glycans are “indirect” and lack the required sensitivity to validate probiotic-prebiotic application. To address these limitations, I have extended fluorescent glycan conjugates (FGCs) to visualize polysaccharide uptake in bacterial isolates and complex ecosystems at the single-cell level. Yeast α-mannan (YM) was conjugated to 6-aminofluorescein and fed to pure cultures of Bacteroides thetaiotaomicron VPI-5482, a well-studied intestinal symbiont that metabolizes YM, and closely related bovine-adapted bacterial isolates. Uptake of FGCs, coupled to complementary genomic and transcriptomic analysis, provided direct evidence of individual genotypes endowed with YM metabolic potential in pure and complex culture. en_US
dc.description.sponsorship This work was supported by funding from the Beef and Cattle Research Council (Grant: FDE.13.15 & FDE.14.17). en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciences en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Bacteroides en_US
dc.subject Rumen en_US
dc.subject Polysaccharide Utilization Loci en_US
dc.subject Microbiome en_US
dc.subject Fluorescent Glycan Conjugates en_US
dc.subject Glycoside Hydrolase en_US
dc.subject Yeast Mannan en_US
dc.subject Carbohydrate en_US
dc.subject Dissertations, Academic en_US
dc.title Direct visualization of yeast mannan metabolism in bovine-adapted Bacteroides thetaiotaomicron strains at the single cell level using fluoresceinamine - yeast mannan conjugates en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.degree.level Masters en_US
dc.proquest.subject 0410 en_US
dc.proquest.subject 0307 en_US
dc.proquest.subject 0715 en_US
dc.proquestyes Yes en_US


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