Direct visualization of yeast mannan metabolism in bovine-adapted Bacteroides thetaiotaomicron strains at the single cell level using fluoresceinamine - yeast mannan conjugates

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dc.contributor.authorKlassen, Leeann
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorAbbott, D. Wade
dc.contributor.supervisorYevtushenko, Dmytro P.
dc.date.accessioned2020-01-22T22:14:47Z
dc.date.available2020-01-22T22:14:47Z
dc.date.issued2019
dc.degree.levelMastersen_US
dc.description.abstractThe rumen of beef cattle houses a diverse community of microorganisms that impact feed digestion, nutrient accessibility, host health, and waste production. Fibrolytic enzymes, probiotics, and prebiotics are promising candidates for next-generation feed additives, with the goal of inducing beneficial changes to the rumen microbiome. Current methods to investigate the mechanistic interactions between bacteria and feed glycans are “indirect” and lack the required sensitivity to validate probiotic-prebiotic application. To address these limitations, I have extended fluorescent glycan conjugates (FGCs) to visualize polysaccharide uptake in bacterial isolates and complex ecosystems at the single-cell level. Yeast α-mannan (YM) was conjugated to 6-aminofluorescein and fed to pure cultures of Bacteroides thetaiotaomicron VPI-5482, a well-studied intestinal symbiont that metabolizes YM, and closely related bovine-adapted bacterial isolates. Uptake of FGCs, coupled to complementary genomic and transcriptomic analysis, provided direct evidence of individual genotypes endowed with YM metabolic potential in pure and complex culture.en_US
dc.description.sponsorshipThis work was supported by funding from the Beef and Cattle Research Council (Grant: FDE.13.15 & FDE.14.17).en_US
dc.identifier.urihttps://hdl.handle.net/10133/5671
dc.language.isoen_USen_US
dc.proquest.subject0410en_US
dc.proquest.subject0307en_US
dc.proquest.subject0715en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectBacteroidesen_US
dc.subjectRumenen_US
dc.subjectPolysaccharide Utilization Locien_US
dc.subjectMicrobiomeen_US
dc.subjectFluorescent Glycan Conjugatesen_US
dc.subjectGlycoside Hydrolaseen_US
dc.subjectYeast Mannanen_US
dc.subjectCarbohydrateen_US
dc.subjectDissertations, Academicen_US
dc.titleDirect visualization of yeast mannan metabolism in bovine-adapted Bacteroides thetaiotaomicron strains at the single cell level using fluoresceinamine - yeast mannan conjugatesen_US
dc.typeThesisen_US
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