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dc.contributor.supervisor Golsteyn, Roy
dc.contributor.author Tuescher, Jan M.
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2019-05-29T20:19:52Z
dc.date.available 2019-05-29T20:19:52Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/10133/5371
dc.description.abstract In this thesis, we investigate the prairie plant species Symphoricarpos occidentalis for compounds of biological interest. We have characterized the effects of S. occidentalis extracts in different human cell lines by phenotypic assays and found that they induce a light dependent vacuolation of the endoplasmic reticulum and the nuclear envelope. Vacuolated cells show an intense lamin A/C signal, in particular at the location of the nuclear envelope derived vacuole. We found that vacuolation appeared independent of protein synthesis, vacuolated cells were alive at 16 h, and light co-treatment increased extract toxicity 10-fold by 96 h. Spectral analyses and phenotypic screening showed that the cyclic tetrapyrrole compound, Pheophorbide a, had a similar effect on human cells. Insight into cytoplasmic vacuolation might be beneficial as a tool to study nuclear envelope organization and contribute to the understanding of lamin-related diseases, such as Pelger-Huët anomaly. en_US
dc.language.iso en_US en_US
dc.publisher Lethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciences en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject vacuole en_US
dc.subject vacuolation en_US
dc.subject photosensitizer en_US
dc.subject human cells en_US
dc.subject Pheophorbide a en_US
dc.subject Symphoricarpos occidentalis en_US
dc.subject Western snowberry -- Therapeutic use -- Research en_US
dc.subject Medicinal plants -- Research en_US
dc.subject Materia medica, Vegetable en_US
dc.subject Botany, Medical -- Research en_US
dc.subject Pharmacognosy en_US
dc.subject Plant extracts -- Therapeutic use -- Research en_US
dc.subject Plant metabolites en_US
dc.subject Microbiological assay en_US
dc.subject Dissertations, Academic en_US
dc.title Extracts from the prairie plant Symphoricarpos occidentalis or the natural product, Pheophorbide a, induce light-dependent vacuolation of human cells en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.degree.level Masters en_US
dc.proquest.subject 0379 en_US
dc.proquest.subject 0307 en_US
dc.proquest.subject 0306 en_US
dc.proquestyes Yes en_US
dc.embargo No en_US


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