Extracts from the prairie plant Symphoricarpos occidentalis or the natural product, Pheophorbide a, induce light-dependent vacuolation of human cells

Simple item page
dc.contributor.authorTuescher, Jan M.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorGolsteyn, Roy
dc.date.accessioned2019-05-29T20:19:52Z
dc.date.available2019-05-29T20:19:52Z
dc.date.issued2018
dc.degree.levelMastersen_US
dc.description.abstractIn this thesis, we investigate the prairie plant species Symphoricarpos occidentalis for compounds of biological interest. We have characterized the effects of S. occidentalis extracts in different human cell lines by phenotypic assays and found that they induce a light dependent vacuolation of the endoplasmic reticulum and the nuclear envelope. Vacuolated cells show an intense lamin A/C signal, in particular at the location of the nuclear envelope derived vacuole. We found that vacuolation appeared independent of protein synthesis, vacuolated cells were alive at 16 h, and light co-treatment increased extract toxicity 10-fold by 96 h. Spectral analyses and phenotypic screening showed that the cyclic tetrapyrrole compound, Pheophorbide a, had a similar effect on human cells. Insight into cytoplasmic vacuolation might be beneficial as a tool to study nuclear envelope organization and contribute to the understanding of lamin-related diseases, such as Pelger-Huët anomaly.en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5371
dc.language.isoen_USen_US
dc.proquest.subject0379en_US
dc.proquest.subject0307en_US
dc.proquest.subject0306en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectvacuoleen_US
dc.subjectvacuolationen_US
dc.subjectphotosensitizeren_US
dc.subjecthuman cellsen_US
dc.subjectPheophorbide aen_US
dc.subjectSymphoricarpos occidentalisen_US
dc.subjectWestern snowberry -- Therapeutic use -- Researchen_US
dc.subjectMedicinal plants -- Researchen_US
dc.subjectMateria medica, Vegetableen_US
dc.subjectBotany, Medical -- Researchen_US
dc.subjectPharmacognosyen_US
dc.subjectPlant extracts -- Therapeutic use -- Researchen_US
dc.subjectPlant metabolitesen_US
dc.subjectMicrobiological assayen_US
dc.subjectDissertations, Academicen_US
dc.titleExtracts from the prairie plant Symphoricarpos occidentalis or the natural product, Pheophorbide a, induce light-dependent vacuolation of human cellsen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
TUESCHER_JAN_MSC_2018.pdf
Size:
8.12 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
3.25 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Arts and Science, Faculty of
University of Lethbridge Theses