Wieden, Hans-Joachim

 

Recent Submissions

  • Juranek, Stefan; Wieden, Hans-Joachim; Lipps, Hans J. (Oxford University PressArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Witten/HerdeckeUniversity of Lethbridgehttps://doi.org/10.1093/nar/gkg233, 2003)
    Dramatic DNA reorganization and elimination processes occur during macronuclear differentiation in ciliates. In this study we analyzed whether cytosine methylation of specific sequences plays a functional role during DNA ...
  • Gu, Shan-Qing; Peske, Frank; Wieden, Hans-Joachim; Rodnina, Marina V.; Wintermeyer, Wolfgang (Cold Springs Harbor Laboratory PressArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Witten/HerdeckeUniversity of Lethbridge, 2003)
    The signal recognition particle (SRP) from Escherichia coli, composed of Ffh protein and 4.5S RNA, mediates membrane targeting of translating ribosomes displaying a signal or signal-anchor sequence. SRP binds at the peptide ...
  • Beal, Jacob; Haddock-Angelli, Traci; Gershater, Markus; De Mora, Kim; Lizarazo, Meagan; Hollenhorst, Jim; Rettberg, Randy; iGEM Interlab Study Contributors (Public Library of ScienceArts and ScienceDepartment of Chemistry and BiochemistryRaytheon BBN TechnologiesiGEM FoundationSynthaceAgilentUniversity of Lethbridge, 2016)
    We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions ...
  • De Laurentiis, Evelina I.; Girodat, Dylan J.; Mercier, Evan; Wieden, Hans-Joachim (Biomath ForumArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Lethbridge, 2016)
    Translation, the ribosome dependent synthesis of proteins, is the last step of gene expression. It is targeted by a large number of antibiotics that modulate, in one way or the other, the dynamic properties of the involved ...
  • Tillault, Anne-Sophie; Fourmann, Jean-Baptiste; Loegler, Christine; Wieden, Hans-Joachim; Wieden-Kothe, Ute; Charpentier, Bruno (Cold Spring Harbor Laboratory PressArts and ScienceDepartment of Chemistry and BiochemistryBipole de l'Université de LorraineUniversity of Lethbridge, 2015)
    In all organisms, several distinct stand-alone pseudouridine synthase (PUS) family enzymes are expressed to isomerizeuridine into pseudouridine (Ψ) by specific recognition of RNAs. In addition, Ψs are generated in Archaea ...
  • Gzyl, Katherine E.; Wieden, Hans-Joachim (Public Library of ScienceArts and ScienceDepartment of Chemistry and BiochemistryUniversity of Lethbridge, 2017)
    Understanding the molecular mechanism of antibiotics that are currently in use is important for the development of new antimicrobials. The tetracyclines, discovered in the 1940s, are a well-establis ...
  • Friedt, Jenna; Leavens, Fern M. V.; Mercier, Evan; Wieden, Hans-Joachim; Wieden-Kothe, Ute (Oxford University PressArts and ScienceDepartment of Chemistry and BiochemistryMax Planck Institute for Biophysical ChemistryUniversity of Lethbridge, 2014)
    Pseudouridine synthases introduce the most common RNA modification and likely use the same catalytic mechanism. Besides a catalytic aspartate residue, the contributions of other residues for catalysis of pseudouridine ...
  • Coatham, Mackenzie L.; Brandon, Harland E.; Fischer, Jeffrey J.; Schummer, Tobias; Wieden, Hans-Joachim (Oxford University PressArts and ScienceDepartment of Chemistry and BiochemistryUniversity of AlbertaUniversity of Lethbridgehttps://doi.org/10.1093/nar/gkv1524, 2016)
    Using a combination of biochemical, structural probing and rapid kinetics techniques we reveal for the first time that the universally conserved translational GTPase (trGTPase) HflX binds to the E-site of the 70S ribosome ...