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dc.contributor.supervisor Abbott, D. Wade
dc.contributor.supervisor Mosimann, Steven C.
dc.contributor.author Yamashita, Justin
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2016-04-11T17:40:41Z
dc.date.available 2016-04-11T17:40:41Z
dc.date.issued 2016
dc.identifier.uri https://hdl.handle.net/10133/4462
dc.description.abstract Dietary fibre fermentation produces short-chain fatty acids, including butyrate (NaB). NaB increases histone tail acetylation within the chromatin of colonic cells, a process associated with increased gene expression. Campylobacter jejuni a prominent foodborne pathogen triggers inflammatory enteritis, whereas NaB and the commensal enteric bacterium Bacteroides thetaiotaomicron (B. theta) have documented anti-inflammatory properties. In this study I have developed a model system for studying the NaB-colonocyte-bacteria interaction and have used it to investigate responses to NaB, C. jejuni and B. theta using an in-house immunomodulatory gene array. NaB induced significant levels of transcription for nearly every immunomodulatory gene. Further, NaB and B. theta stimulate the expression of defensins and Toll-like receptors, while C. jejuni decreased both. This study provides mechanistic insights into the human colonocyte immune response and presents several gene targets as potential biomarkers for inflammatory expression responses in vivo. en_US
dc.description.sponsorship Alberta Innovates Bio Solutions (AIBS) and Alberta Livestock and Meat Agency Ltd (ALMA) en_US
dc.language.iso en_CA en_US
dc.publisher Lethbridge, Alta : University of Lethbridge, Dept. of Chemistry and Biochemistry en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject Caco-2 en_US
dc.subject butyrate en_US
dc.subject butyric acid en_US
dc.subject H3K9 en_US
dc.subject Microflora en_US
dc.subject Inflammation en_US
dc.subject Campylobacter jejuni en_US
dc.subject Bacteroides thetaiotaomicron en_US
dc.subject defensin en_US
dc.subject Toll-like receptor en_US
dc.title Butyrate, Bacteroides thetaiotaomicron and Campylobacter jejuni modulate the expression of beta-defensins, Toll-like receptors and cytokines in Caco-2 cells en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Chemistry & Biochemistry en_US
dc.degree.level Masters en_US
dc.proquest.subject 0487 en_US
dc.proquest.subject 0410 en_US
dc.proquest.subject 0982 en_US
dc.proquestyes Yes en_US
dc.embargo No en_US


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