Butyrate, Bacteroides thetaiotaomicron and Campylobacter jejuni modulate the expression of beta-defensins, Toll-like receptors and cytokines in Caco-2 cells

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dc.contributor.authorYamashita, Justin
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorAbbott, D. Wade
dc.contributor.supervisorMosimann, Steven C.
dc.date.accessioned2016-04-11T17:40:41Z
dc.date.available2016-04-11T17:40:41Z
dc.date.issued2016
dc.degree.levelMastersen_US
dc.description.abstractDietary fibre fermentation produces short-chain fatty acids, including butyrate (NaB). NaB increases histone tail acetylation within the chromatin of colonic cells, a process associated with increased gene expression. Campylobacter jejuni a prominent foodborne pathogen triggers inflammatory enteritis, whereas NaB and the commensal enteric bacterium Bacteroides thetaiotaomicron (B. theta) have documented anti-inflammatory properties. In this study I have developed a model system for studying the NaB-colonocyte-bacteria interaction and have used it to investigate responses to NaB, C. jejuni and B. theta using an in-house immunomodulatory gene array. NaB induced significant levels of transcription for nearly every immunomodulatory gene. Further, NaB and B. theta stimulate the expression of defensins and Toll-like receptors, while C. jejuni decreased both. This study provides mechanistic insights into the human colonocyte immune response and presents several gene targets as potential biomarkers for inflammatory expression responses in vivo.en_US
dc.description.sponsorshipAlberta Innovates Bio Solutions (AIBS) and Alberta Livestock and Meat Agency Ltd (ALMA)en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/4462
dc.language.isoen_CAen_US
dc.proquest.subject0487en_US
dc.proquest.subject0410en_US
dc.proquest.subject0982en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta : University of Lethbridge, Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry & Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectCaco-2en_US
dc.subjectbutyrateen_US
dc.subjectbutyric aciden_US
dc.subjectH3K9en_US
dc.subjectMicrofloraen_US
dc.subjectInflammationen_US
dc.subjectCampylobacter jejunien_US
dc.subjectBacteroides thetaiotaomicronen_US
dc.subjectdefensinen_US
dc.subjectToll-like receptoren_US
dc.titleButyrate, Bacteroides thetaiotaomicron and Campylobacter jejuni modulate the expression of beta-defensins, Toll-like receptors and cytokines in Caco-2 cellsen_US
dc.typeThesisen_US
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