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dc.contributor.supervisor Schultz, Elizabeth
dc.contributor.author Erickson, Jessica
dc.contributor.author University of Lethbridge. Faculty of Arts and Science
dc.date.accessioned 2016-02-27T18:17:47Z
dc.date.available 2016-02-27T18:17:47Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/10133/4433
dc.description.abstract The plant hormone auxin is essential for cell differentiation into vascular tissue, and leaf cells that will become veins show asymmetric distribution of the plasma membrane protein PINFORMED1 (PIN1), which directs auxin flux. The formation, sorting and transport of PIN1 vesicles within the endomembrane system leads to a high concentration of PIN1 on one membrane face. Many proteins involved in directing PIN1 to target membranes, including FORKED1 (FKD1). A yeast two-hybrid cDNA library screen was performed to identify protein interacters that might provide information about the molecular mechanism of FKD1. Results suggested that FKD1 is capable of forming homodimers through its DUF828 domain, but that this interaction depends on the absence of the 57 N-terminal amino acids. A second protein interacter was identified, ARFA1e, but later found to be a false positive. Despite the lack of interaction with FKD1, arfa1e-1 mutants displayed subtle vascular cotyledon phenotype characterized by increased vein number and increased vein meeting. The arfa1e-2 allele and mutants closely related genes (arfa1a and arfa1b) displayed similar defects. Double mutants showed an increase in phenotypic severity, supporting the notion that these proteins are acting redundantly during establishment of cotyledon vein pattern. en_US
dc.language.iso en_CA en_US
dc.publisher Lethbridge, Alta. : University of Lethbridge, Department of Biological Sciences en_US
dc.relation.ispartofseries Thesis (University of Lethbridge. Faculty of Arts and Science) en_US
dc.subject auxin en_US
dc.subject homodimers en_US
dc.subject cotyledon vein pattern en_US
dc.subject plasma membrane protein en_US
dc.subject PINFORMED1 en_US
dc.subject FORKED1 en_US
dc.subject DUF828 en_US
dc.subject ARFA1e protein en_US
dc.subject amino acids en_US
dc.subject vascular patterning en_US
dc.title A yeast two-hybrid screen in Arabidopsis thaliana suggests FORKED1 forms homodimers and the ARFA1 family has a role in cotyledon vein patterning en_US
dc.type Thesis en_US
dc.publisher.faculty Arts and Science en_US
dc.publisher.department Department of Biological Sciences en_US
dc.degree.level Masters en_US
dc.proquestyes No en_US
dc.embargo No en_US


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