A yeast two-hybrid screen in Arabidopsis thaliana suggests FORKED1 forms homodimers and the ARFA1 family has a role in cotyledon vein patterning

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dc.contributor.authorErickson, Jessica
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorSchultz, Elizabeth
dc.date.accessioned2016-02-27T18:17:47Z
dc.date.available2016-02-27T18:17:47Z
dc.date.issued2011
dc.degree.levelMastersen_US
dc.description.abstractThe plant hormone auxin is essential for cell differentiation into vascular tissue, and leaf cells that will become veins show asymmetric distribution of the plasma membrane protein PINFORMED1 (PIN1), which directs auxin flux. The formation, sorting and transport of PIN1 vesicles within the endomembrane system leads to a high concentration of PIN1 on one membrane face. Many proteins involved in directing PIN1 to target membranes, including FORKED1 (FKD1). A yeast two-hybrid cDNA library screen was performed to identify protein interacters that might provide information about the molecular mechanism of FKD1. Results suggested that FKD1 is capable of forming homodimers through its DUF828 domain, but that this interaction depends on the absence of the 57 N-terminal amino acids. A second protein interacter was identified, ARFA1e, but later found to be a false positive. Despite the lack of interaction with FKD1, arfa1e-1 mutants displayed subtle vascular cotyledon phenotype characterized by increased vein number and increased vein meeting. The arfa1e-2 allele and mutants closely related genes (arfa1a and arfa1b) displayed similar defects. Double mutants showed an increase in phenotypic severity, supporting the notion that these proteins are acting redundantly during establishment of cotyledon vein pattern.en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/4433
dc.language.isoen_CAen_US
dc.proquestyesNoen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectauxinen_US
dc.subjecthomodimersen_US
dc.subjectcotyledon vein patternen_US
dc.subjectplasma membrane proteinen_US
dc.subjectPINFORMED1en_US
dc.subjectFORKED1en_US
dc.subjectDUF828en_US
dc.subjectARFA1e proteinen_US
dc.subjectamino acidsen_US
dc.subjectvascular patterningen_US
dc.titleA yeast two-hybrid screen in Arabidopsis thaliana suggests FORKED1 forms homodimers and the ARFA1 family has a role in cotyledon vein patterningen_US
dc.typeThesisen_US
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