Immediate-early gene Homer1a intranuclear transcription focus intensity as a measure of relative neural activation

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dc.contributor.authorLi Witharana, Wing K.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorMcNaughton, Bruce L.
dc.date.accessioned2019-06-19T16:01:50Z
dc.date.available2019-06-19T16:01:50Z
dc.date.issued2018
dc.degree.levelPh.Den_US
dc.description.abstractAlthough immediate-early gene expression analyses using fluorescent in situ hybridization is an effective method to identify recently activated neurons; and non-Boolean variations in transcription foci have been documented, it remains unclear whether there is a systematic relationship between magnitude of neural activation and corresponding RNA signal. Here, we quantified the Homer1a response of hippocampal neurons in rats that ran laps on a familiar track to induce consistent cell firing. A strong linear trend (r2 > 0.9) in INF intensity (brightness) was observed between 1 and 25 laps, after which INF signal dispersed within the nucleus. When the integrated intranuclear fluorescence was considered instead, the linear relationship extended to 50 laps. But there was only an approximate doubling of Homer1a RNA detected for this 50-fold variation in total spiking. Thus, this low-gain dynamic range likely precludes INF intensity as a precise quantitative readout of neural activation, albeit a useful qualitative tool.en_US
dc.description.sponsorshipAlberta Innovates - Health Solutions: Polaris Award Natural Sciences and Engineering Research Council of Canada: RGPIN-2017-03857 National Science Foundation: 1631465en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5404
dc.language.isoen_USen_US
dc.proquest.subject0317en_US
dc.proquestyesYesen_US
dc.publisher.departmentDepartment of Neuroscienceen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectNeurons -- Researchen_US
dc.subjectHippocampus (Brain) -- Researchen_US
dc.subjectTranscription factors -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.subjectelectro-transcriptional couplingen_US
dc.subjecthippocampusen_US
dc.subjectHomer1aen_US
dc.subjectimmediate-early geneen_US
dc.subjecttranscriptionen_US
dc.subjectGenetic transcription -- Researchen_US
dc.titleImmediate-early gene Homer1a intranuclear transcription focus intensity as a measure of relative neural activationen_US
dc.typeThesisen_US
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