The potential molecular mechanisms of various treatment combinations between cisplatin, cannabidiol, cannabis extracts, and intermittent serum starvation on colorectal cancer cell lines
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Date
2023-05-01
Authors
Cherkasova, Viktoriia
University of Lethbridge. Faculty of Arts and Science
Journal Title
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Publisher
Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences
Abstract
Colorectal cancer is the third most common cancer worldwide. The mortality rates remain high despite the wide variety of multiple preventive and treatment options.
Platinum-derived chemotherapy medications are often combined with other conventional therapies for treating different tumors, including colorectal cancer. However, the development of drug resistance and multiple adverse effects remain common in clinical settings. Thus, there is a necessity to find novel treatments and drug combinations that could effectively target colorectal cancer cells without affecting normal tissues and lower the probability of disease relapse.
To find potential synergistic interaction, I tried multiple different combinations between cisplatin, cannabidiol, cannabis extracts, and intermittent serum starvation on colorectal cancer cell lines. Based on the cell viability assay, we found that combinations between cannabidiol and intermittent serum starvation, cisplatin and intermittent serum starvation, as well as cisplatin, cannabidiol and intermittent serum starvation, can work in a synergistic fashion on different colorectal cancer cell lines. I analyzed differentially expressed genes and affected pathways in two colorectal cancer cell lines to further understand the potential molecular mechanisms behind the treatments and their interactions.
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Keywords
Colorectal cancer , Cancer cell lines , Cannabidiol , Cannabis extracts , Intermittent serum starvation , Synergistic interactions