Spliceosomal intron and spliceosome evolution in Giardia lamblia and other diplomonads

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dc.contributor.authorHudson, Andrew J.
dc.contributor.supervisorRussell, Anthony G.
dc.contributor.supervisorGoater, Cameron P.
dc.date.accessioned2015-01-15T21:00:36Z
dc.date.available2015-01-15T21:00:36Z
dc.date.issued2014
dc.degree.levelPh.Den_US
dc.description.abstractSpliceosomal introns interrupt protein coding genes in all characterized eukaryotic nuclear genomes and are removed by a large RNA-protein complex termed the spliceosome. Diplomonads are diverse unicellular eukaryotes that display compact genomes with few spliceosomal introns. My thesis objectives were to explore spliceosomal intron and spliceosome diversity as well as RNA processing mechanisms in the diplomonads Giardia lamblia and Spironucleus spp. Surprisingly, G. lamblia was found to contain a proportionally large number of fragmented spliceosomal introns that are spliced in trans from separate pre-mRNA molecules. Next, both evolutionarily divergent and conventional spliceosomal small nuclear RNAs were identified in G. lamblia and Spironucleus spp. and an RNA 3สน end motif was determined to be involved in processing of both non-coding RNAs and trans-introns in G. lamblia. These findings shed light on spliceosome and spliceosomal intron evolution in eukaryotes undergoing severe genomic reduction and potentially complete loss of their spliceosomal introns.en_US
dc.description.sponsorshipUniversity of Lethbridge (SGS Graduate Fellowship), Natural Sciences and Engineering Research Council of Canada (NSERC) (Discovery Grant and Alexander Graham Bell Canada Graduate Scholarships).en_US
dc.identifier.urihttps://hdl.handle.net/10133/3615
dc.language.isoenen_US
dc.proquest.subject0307en_US
dc.proquest.subject0715en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Scienceen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectSpliceosomal intronsen_US
dc.subjectSpliceosomeen_US
dc.subjectNon-coding RNAen_US
dc.subjectEvolutionen_US
dc.subjectProtisten_US
dc.subjectGiardia lambliaen_US
dc.subjectSpironucleusen_US
dc.subjectDiplomonaden_US
dc.subjectRNA processingen_US
dc.titleSpliceosomal intron and spliceosome evolution in Giardia lamblia and other diplomonadsen_US
dc.typeThesisen_US
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