Development of a real-time immuno-PCR assay for the quantification of environmental contaminants
dc.contributor.author | Gaudet, Daniel | |
dc.contributor.supervisor | Sheedy, Claudia | |
dc.contributor.supervisor | Hontela, Alice | |
dc.date.accessioned | 2015-01-29T19:03:15Z | |
dc.date.available | 2015-01-29T19:03:15Z | |
dc.date.issued | 2014 | |
dc.degree.level | Masters | en_US |
dc.degree.level | Masters | |
dc.description.abstract | This thesis outlines the development of two universal real-time immuno-PCR (RT-iPCR) assays for application in agriculture. The first RT-iPCR was developed for the sensitive quantification of 17β-estradiol in water. Using a universal iPCR method and polyclonal antibodies, 17β-estradiol was accurately quantified at concentrations ranging from 1 pg mL-1 to 10 μg mL-1, with a limit of detection of 0.7 pg mL-1. The RT-iPCR assay provided an 800-fold increase in sensitivity as well as an expanded working range compared to the corresponding enzyme-linked immunosorbent assay. Antibodies were swapped with antibodies specific to P. brassicae for the quantification of clubroot resting spores using the same RT-iPCR assay. P. brassicae resting spores were quantified in the range of 50 to 10 000 spores, with a detection limit of 29 spores. Both RT-iPCR showed equal or improved sensitivity compared to other published analytical methods, with expanded linear working ranges and high-throughput capabilities. | en_US |
dc.description.sponsorship | Agriculture and Agri-Foods Canada | en_US |
dc.identifier.uri | https://hdl.handle.net/10133/3629 | |
dc.language.iso | en_CA | en_US |
dc.proquest.subject | 0473 | en_US |
dc.proquest.subject | 0486 | en_US |
dc.proquest.subject | 0307 | en_US |
dc.proquestyes | Yes | en_US |
dc.publisher | Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences | en_US |
dc.publisher.department | Department of Biological Sciences | en_US |
dc.publisher.faculty | Arts and Science | en_US |
dc.relation.ispartofseries | Thesis (University of Lethbridge. Faculty of Arts and Science) | en_US |
dc.subject | Immunodiagnostic development for agricultural contaminants | en_US |
dc.title | Development of a real-time immuno-PCR assay for the quantification of environmental contaminants | en_US |
dc.type | Thesis | en_US |