Regulation of mRNA translation by eIF5B in head and neck squamous cell carcinoma (HNSCC)

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dc.contributor.authorMuley, Rachana
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorThakor, Nehal
dc.date.accessioned2020-05-12T16:27:33Z
dc.date.available2020-05-12T16:27:33Z
dc.date.issued2020
dc.degree.levelMastersen_US
dc.description.abstractStudies have demonstrated that eIF5B promotes cap-independent translation of the anti-apoptotic factor, XIAP, under stress conditions. Also, eIF5B depletion results in the decreased levels of several pro-survival proteins, thus sensitizing glioblastoma cells to TRAIL-induced apoptosis. Here, we have shown high EIF5B mRNA expression in HNSCC was associated with poor patients’ survival. Further, to assess the impact of eIF5B on HNSCC biology, we depleted eIF5B in three HNSCC cell lines. We also show that eIF5B depletion enhanced the sensitivity of Cal-33 and UM-SCC-29 cells to TRAIL-induced apoptosis. Further, we show the levels of anti-apoptotic proteins Bcl-xL, cIAP1, cFLIPs, and XAIP were decreased upon eIF5B depletion in Cal-33 suggesting the role of eIF5B in the translation of these proteins which was further supported by our polysome profiling data. Altogether our findings suggest that eIF5B may play an important role in the translation of mRNAs encoding key anti-apoptotic proteins that evade apoptosis.en_US
dc.identifier.urihttps://hdl.handle.net/10133/5728
dc.language.isoen_USen_US
dc.proquest.subjectBiochemistry [0487]en_US
dc.proquest.subjectOncology [0992]en_US
dc.proquest.subjectCellular biology [0379]en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry & Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry & Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectCancer -- Gene therapyen_US
dc.subjectCancer -- Treatmenten_US
dc.subjectCancer genesen_US
dc.subjectGlioblastoma multiformeen_US
dc.subjectSquamous cell carcinomaen_US
dc.titleRegulation of mRNA translation by eIF5B in head and neck squamous cell carcinoma (HNSCC)en_US
dc.typeThesisen_US
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