Catabolism of structurally diverse fructans by bovine-adapted Bifidobacterium and Lactobacillus species in pure culture and ex vivo rumen microbial communities

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dc.contributor.authorKing, Marissa L. R.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorAbbot, D. Wade
dc.contributor.supervisorPatel, Trushar R.
dc.date.accessioned2022-12-09T19:32:33Z
dc.date.available2022-12-09T19:32:33Z
dc.date.issued2022
dc.degree.levelMastersen_US
dc.description.abstractInulin and inulin-derived fructooligosaccharides (FOS) are well-known prebiotics for use in monogastric animals, but their potency in ruminants has not been determined. Additionally, structurally diverse fructans, such as graminan-type fructans purified from immature cereal crops may also possess prebiotic potential. As the composition of the rumen microbiome is closely associated with traits, such as: feed utilization, health, and waste production; prebiotics and probiotics are promising additives that can shift the microbial community towards a more productive state. In this thesis, the ability of bovine-adapted Bifidobacterium and Lactobacillus species to utilize FOS, inulin, levan, and graminans was assessed. Fluorescently labelled fructans and FISH were performed to directly visualize fructan interactions with Bifidobacterium and Lactobacillus species at the single-cell level. The prebiotic potential of inulin was compared between naïve or inulin-adapted microbial communities. This research provides new methods and benchmarks for evaluating fructans derived from diverse sources as prebiotic candidates in cattle.en_US
dc.identifier.urihttps://hdl.handle.net/10133/6390
dc.language.isoen_USen_US
dc.proquest.subject0410en_US
dc.proquest.subject0487en_US
dc.proquest.subject0475en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Dept. of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectrumenen_US
dc.subjectprebioticen_US
dc.subjectinulinen_US
dc.subjectfructanen_US
dc.subjectRumen--Microbiology--Researchen_US
dc.subjectPrebiotics--Researchen_US
dc.subjectInulin--Researchen_US
dc.subjectFructans--Researchen_US
dc.subjectRuminants--Digestive organsen_US
dc.subjectRuminants--Feeding and feeds--Researchen_US
dc.subjectRuminants--Nutritionen_US
dc.subjectFeeds--Microbiologyen_US
dc.subjectCattle--Nutritionen_US
dc.subjectCattle--Feeding and feeds--Researchen_US
dc.subjectCattle--Metabolismen_US
dc.subjectBacteria--Health aspectsen_US
dc.subjectGastrointestinal system--Microbiologyen_US
dc.subjectCereal grasses--Researchen_US
dc.subjectMetabolism--Researchen_US
dc.subjectFeed additives--Researchen_US
dc.subjectDissertations, Academicen_US
dc.titleCatabolism of structurally diverse fructans by bovine-adapted Bifidobacterium and Lactobacillus species in pure culture and ex vivo rumen microbial communitiesen_US
dc.typeThesisen_US
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