Nuclear magnetic resonance spectroscopic characterization of peptide based nanocarriers and cargo complexes

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dc.contributor.authorBanwo, Inumidun Damilola
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorEudes, François
dc.contributor.supervisorHazendonk, Paul
dc.date.accessioned2018-08-07T21:17:39Z
dc.date.available2018-08-07T21:17:39Z
dc.date.issued2018
dc.degree.levelMastersen_US
dc.description.abstractCell penetrating peptides (CPPs) are short peptide 8-30 amino acids polypeptides that can deliver various molecules (nanoparticles to large fragment DNA) to subcellular locations such as the plastid organelles, cytoplasm, and nucleus etc. CPPs are characterized by containing clusters of cationic side chains that allow them to interact directly with the polar membrane surface, which enables then to enter the cell. One of the function of CPPs are to deliver cargo molecules such as DNA and RNA into cells. Most application have been developed for animal cells; however, the use of CPPs in plant cells remain a small researched field because of insufficient understanding of their mode of uptake. The present research describes firstly, the use of NMR technique to elucidate the structural properties of CPP-DNA complexation. Secondly the dynamic of the complexation. Finally, to relate the observations to their translocation mechanism. For this study, short peptides such as; arginine (R), tri-arginine (R3), nano-arginine (R9), and Tat2 (RKKRRQRRRRKKRRQRRR) were complexed with single-stranded and double-stranded DNA 5’ AGTCC 3’. The interaction between the single-stranded and double-stranded DNA 5’ AGTCC 3’ with the peptides was compared to understand how different DNA strands can influence complex formation. These complexes are used at relatively high concentrations; hence, measurements were at millimolar concentration. Therefore, for the study of DNA-CPP complexes, both non-isotopically enriched DNA and CPP samples were used. The reason for using such samples is to see if complexation can be observed using NMR spectroscopy, which will reduce the use of costly materials.en_US
dc.description.sponsorshipUniversity of Lethbridge, Agric and Agricultural Food Canadaen_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5180
dc.language.isoen_USen_US
dc.proquest.subject0487en_US
dc.proquest.subject0379en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Chemistry and Biochemistryen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectCells -- Permeability -- Researchen_US
dc.subjectPlant cell membranes -- Researchen_US
dc.subjectNuclear magnetic resonance spectroscopyen_US
dc.subjectBiological transport -- Physiologyen_US
dc.subjectPeptides -- Phsyiological transporten_US
dc.subjectcargo moleculesen_US
dc.subjectcell penetrating peptidesen_US
dc.subjectcellular uptakeen_US
dc.subjectNMR spectroscopyen_US
dc.subjectplant cellsen_US
dc.subjectplasma membrane passageen_US
dc.titleNuclear magnetic resonance spectroscopic characterization of peptide based nanocarriers and cargo complexesen_US
dc.typeThesisen_US
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