Bioinformatic analysis of structure and function of LIM domains of human zyxin family proteins

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dc.contributor.authorSiddiqui, M. Quadir
dc.contributor.authorBadmalia, Maulik D.
dc.contributor.authorPatel, Trushar R.
dc.date.accessioned2022-10-31T20:04:56Z
dc.date.available2022-10-31T20:04:56Z
dc.date.issued2021
dc.descriptionOpen access article. Creative Commons Attribution 4.0 License (CC BY 4.0) appliesen_US
dc.description.abstractMembers of the human Zyxin family are LIM domain-containing proteins that perform critical cellular functions and are indispensable for cellular integrity. Despite their importance, not much is known about their structure, functions, interactions and dynamics. To provide insights into these, we used a set of in-silico tools and databases and analyzed their amino acid sequence, phylogeny, post-translational modifications, structure-dynamics, molecular interactions, and functions. Our analysis revealed that zyxin members are ohnologs. Presence of a conserved nuclear export signal composed of LxxLxL/LxxxLxL consensus sequence, as well as a possible nuclear localization signal, suggesting that Zyxin family members may have nuclear and cytoplasmic roles. The molecular modeling and structural analysis indicated that Zyxin family LIM domains share similarities with transcriptional regulators and have positively charged electrostatic patches, which may indicate that they have previously unanticipated nucleic acid binding properties. Intrinsic dynamics analysis of Lim domains suggest that only Lim1 has similar internal dynamics properties, unlike Lim2/3. Furthermore, we analyzed protein expression and mutational frequency in various malignancies, as well as mapped protein-protein interaction networks they are involved in. Overall, our comprehensive bioinformatic analysis suggests that these proteins may play important roles in mediating protein-protein and protein-nucleic acid interactions.en_US
dc.identifier.citationSiddiqui, M. Q., Badmalia, M. D., & Patel, T. R. (2021). Bioinformatic analysis of structure and funtion of LIM domains of human zyxin family proteins. International Journal of Molecular Sciences, 22(5), Article 2647. https://doi.org/10.3390/ijms22052647en_US
dc.identifier.urihttps://hdl.handle.net/10133/6370
dc.language.isoen_CAen_US
dc.publisherMDPIen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.publisher.institutionUniversity of Lethbridge
dc.publisher.institutionUniversity of Calgary
dc.publisher.institutionUniversity of Alberta
dc.publisher.urlhttps://doi.org/10.3390/ijms22052647
dc.subjectLim domainsen_US
dc.subjectLeucine rich motifsen_US
dc.subjectProtein bioinformaticsen_US
dc.subjectProtein-nucleic acid interactions
dc.subject.lcshZyxin
dc.subject.lcshCancer
dc.subject.lcshProtein-protein interactions
dc.titleBioinformatic analysis of structure and function of LIM domains of human zyxin family proteinsen_US
dc.typeArticleen_US
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