What's in a chicken: using NMR metabolomics to investigate stress in broiler chickens

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dc.contributor.authorBrown, Catherine
dc.contributor.supervisorInglis, Douglas G.
dc.contributor.supervisorSelinger, Brent L.
dc.date.accessioned2022-01-11T19:05:49Z
dc.date.available2022-01-11T19:05:49Z
dc.date.issued2021
dc.degree.levelMastersen_US
dc.description.abstractStress has the potential to adversely affect the health of chickens. Using Nuclear Magnetic Resonance (NMR) spectroscopy-based metabolomics objectives were to: ascertain the effects of physiological stress on the metabolomes of chicken liver, kidney, and breast muscle; compare sample preparation techniques for fecal metabolomics; and determine if NMR spectra can be obtained from feather pulp, and the effects of age and stress on the pulp metabolome. Physiological stress modulated chicken metabolism in all tissues examined, and caused the diversion of energy to muscle catabolism and gluconeogenesis. Ultrafiltration was the most versatile, reproducible, and efficient fecal metabolite extraction method; however, Bligh-Dyer extraction and no extraction with baseline correction produced comparable results. High quality NMR spectra were obtained from chicken feather pulp, and both age and stress significantly altered the pulp metabolome. The results obtained are foundational to the identification of novel biomarkers of stress for use in chicken production settings.en_US
dc.description.sponsorshipFinancial support for chapter 2 was provided in part by grants from Alberta Agriculture and Forestry (2019F101R and 2019H001R), Alberta Chicken Producers, Alberta Innovates (CD-60), the Canadian Glycomics Network (ID-04), and the Canadian Poultry Research Council (Poultry Science Cluster Project 1373 Activity 14). The research in chapter 3 was funded in part by grants from Canadian Institutes of Health Research (CIHR) Project Scheme #363195, Natural Sciences and Engineering Research Council of Cana-da (NSERC) Discovery Grant #5519, Alberta Agriculture and Forestry Grant #2019F101R, and the Canadian Poultry Research Council (CPRC) Poultry Science Cluster Project #1373 Activity 14. The work in chapter 4 was funded in part by grants from the Alberta Agriculture and Forestry (projects 2019F101R and 2019H001R), the Canadian Poultry Research Council (Poultry Science Cluster project ASC-20), and the Canadian Glycomics Network (GlycoNet; project ID-04).en_US
dc.identifier.urihttps://hdl.handle.net/10133/6126
dc.language.isoen_USen_US
dc.proquest.subjectAnimal sciences [0475]en_US
dc.proquest.subjectBiology [0306]en_US
dc.proquest.subjectBiochemistry [0487]en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biologyen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectnuclear magnetic resonance metabolomicsen_US
dc.subjectMetabolites -- Researchen_US
dc.subjectStress (Physiology) -- Researchen_US
dc.subjectBiochemical markers -- Researchen_US
dc.subjectDissertations, Academicen_US
dc.subjectBroilers (Chickens) -- Healthen_US
dc.subjectNuclear magnetic resonanceen_US
dc.titleWhat's in a chicken: using NMR metabolomics to investigate stress in broiler chickensen_US
dc.typeThesisen_US
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