Identification of the mode of regulation of SINE sense and antisense RNAs during cellular response to the stress

Abstract

A large portion of the mammalian genome comprises non-coding sequences, such as B2 SINEs, which play a role in gene regulation during stress. Despite their significance, the regulatory mechanisms governing SINEs remain unclear. This study explores the relationship between sense and antisense B2 SINE transcripts in a mouse cell line model exposed to heat shock and other stress conditions, including transfection with locked nucleic acids and in vitro transcribed B2. The results of qPCR and sequencing data analysis demonstrate an inverse relationship between the expression levels of sense and antisense B2 SINEs, with these effects being transient and diminishing over time. This indicates a complex, dynamic regulatory network that likely involves additional factors or feedback loops.