Current approaches for RNA-labelling to identify RNA-binding proteins

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dc.contributor.authorGemmill, Darren L.
dc.contributor.authorD'souza, Simmone
dc.contributor.authorMeier-Stephenson, Vanessa
dc.contributor.authorPatel, Trushar R.
dc.date.accessioned2023-05-03T18:04:47Z
dc.date.available2023-05-03T18:04:47Z
dc.date.issued2020
dc.descriptionOpen access article. Creative Commons Attribution 4.0 International license (CC BY 4.0) applies
dc.description.abstractRNA is involved in all domains of life, playing critical roles in a host of gene expression processes, host-defense mechanisms, cell proliferation, and diseases. A critical component in many of these events is the ability for RNA to interact with proteins. Over the past few decades, our understanding of such RNA–protein interactions and their importance has driven the search and development of new techniques for the identification of RNA-binding proteins. In determining which proteins bind to the RNA of interest, it is often useful to use the approach where the RNA molecule is the “bait” and allow it to capture proteins from a lysate or other relevant solution. Here, we review a collection of methods for modifying RNA to capture RNA-binding proteins. These include small-molecule modification, the addition of aptamers, DNA-anchoring, and nucleotide substitution. With each, we provide examples of their application, as well as highlight their advantages and potential challenges.
dc.identifier.citationGemmill, D., D'souza, S., Meier-Stephenson, V., & Patel, T. R. (2020). Current approaches for RNA-labelling to identify RNA-binding proteins. Biochemistry and Cell Biology, 98(1), 31-41. https://doi.org/10.1139/bcb-2019-0041
dc.identifier.urihttps://hdl.handle.net/10133/6487
dc.language.isoen
dc.publisherCanadian Science Publishing
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.publisher.institutionUniversity of Lethbridge
dc.publisher.institutionUniversity of Calgary
dc.publisher.institutionUniversity of Alberta
dc.subjectRNA-binding proteins
dc.subjectBiotin labelling
dc.subjectDigoxigenin labelling
dc.subjectAptamer labelling
dc.subjectAffinity capture
dc.subjectNucleotide-substitution method
dc.subject.lcshBiotin
dc.subject.lcshChemical affinity
dc.subject.lcshNucleotides
dc.titleCurrent approaches for RNA-labelling to identify RNA-binding proteins
dc.typeArticle
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