Initial characterization of the functional cycle of YihA

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dc.contributor.authorCapatos, Dora
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden, Hans-Joachim
dc.date.accessioned2023-12-06T21:19:09Z
dc.date.available2023-12-06T21:19:09Z
dc.date.issued2023
dc.degree.levelMasters
dc.description.abstractThe worldwide shortage of new and effective antibiotics is becoming of increasing concern. New antibiotic targets are needed for testing and validation in the development of a new generation of antibiotics. This thesis investigates a potential antibiotic target, the essential GTPase YihA. The objective of this thesis was to gain a greater understanding of the mechanisms that regulate YihA’s functional cycle. Small angle X-ray scattering (SAXS) was used to obtain a low resolution solution structure of YihA and to provide information about the hydrodynamic characteristics of YihA including size, shape and flexibility. Further analysis of the SAXS data by disorder prediction programs and by modelling suggested that YihA in solution contains a folded core with flexible N-terminal and C-terminal tails. The SAXS structure of YihA, the SAXS-based modelling and the solution properties of YihA all supported dimerization of YihA in solution. Additionally, analytical gel filtration chromatography at concentrations of YihA closer to physiological concentrations revealed dimerization of YihA. The characteristics of the dimerization interface in the E. coli YihA crystal structure suggest that dimerization of YihA may play an important role in the functional cycle of YihA. Thus, these studies lay the groundwork for future structural and biochemical studies on the mechanism and significance of dimerization of YihA.
dc.identifier.urihttps://hdl.handle.net/10133/6643
dc.language.isoen
dc.proquestyesNo
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)
dc.subjectAntibiotic target
dc.subjectGTPase
dc.subjectFunctional cycle
dc.subjectYihA
dc.subject.lcshGuanosine triphosphate
dc.subject.lcshDimerization
dc.subject.lcshAntibiotics--Development
dc.subject.lcshDissertations, Academic
dc.titleInitial characterization of the functional cycle of YihA
dc.typeThesis
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