Genomic instability in liver cells caused by an LPS-induced bystander-like effect
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Date
2013
Authors
Kovalchuk, Igor
Walz, Paul
Thomas, James E.
Kovalchuk, Olga
Journal Title
Journal ISSN
Volume Title
Publisher
Public Library of Science
Abstract
Bacterial infection has been linked to carcinogenesis, however, there is lack of knowledge of molecular mechanisms that
associate infection with the development of cancer. We analyzed possible effects of the consumption of heat-killed E. coli
O157:H7 cells or its cellular components, DNA, RNA, protein or lipopolysaccharides (LPS) on gene expression in naı¨ve liver
cells. Four week old mice were provided water supplemented with whole heat-killed bacteria or bacterial components for a
two week period. One group of animals was sacrificed immediately, whereas another group was allowed to consume
uncontaminated tap water for an additional two weeks, and liver samples were collected, post mortem. Liver cells
responded to exposure of whole heat-killed bacteria and LPS with alteration in cH2AX levels and levels of proteins involved
in proliferation, DNA methylation (MeCP2, DNMT1, DNMT3A and 3B) or DNA repair (APE1 and KU70) as well as with changes
in the expression of genes involved in stress response, cell cycle control and bile acid biosynthesis. Other bacterial
components analysed in this study did not lead to any significant changes in the tested molecular parameters. This study
suggests that lipopolysaccharides are a major component of Gram-negative bacteria that induce molecular changes within
naı¨ve cells of the host.
Description
Sherpa Romeo green journal: open access
Keywords
Liver cells , Lipopolysaccharides , LPS , Gene expression , Bacterial infection , Genome instability , Bystander effect
Citation
Kovalchuk, I., Walz, P., Thomas, J., & Kovalchuk, O. (2013). Genomic instability in liver cells caused by an LPS-induced bystander-like effect