Genomic instability in liver cells caused by an LPS-induced bystander-like effect

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dc.contributor.authorKovalchuk, Igor
dc.contributor.authorWalz, Paul
dc.contributor.authorThomas, James E.
dc.contributor.authorKovalchuk, Olga
dc.date.accessioned2016-10-31T18:17:54Z
dc.date.available2016-10-31T18:17:54Z
dc.date.issued2013
dc.descriptionSherpa Romeo green journal: open accessen_US
dc.description.abstractBacterial infection has been linked to carcinogenesis, however, there is lack of knowledge of molecular mechanisms that associate infection with the development of cancer. We analyzed possible effects of the consumption of heat-killed E. coli O157:H7 cells or its cellular components, DNA, RNA, protein or lipopolysaccharides (LPS) on gene expression in naı¨ve liver cells. Four week old mice were provided water supplemented with whole heat-killed bacteria or bacterial components for a two week period. One group of animals was sacrificed immediately, whereas another group was allowed to consume uncontaminated tap water for an additional two weeks, and liver samples were collected, post mortem. Liver cells responded to exposure of whole heat-killed bacteria and LPS with alteration in cH2AX levels and levels of proteins involved in proliferation, DNA methylation (MeCP2, DNMT1, DNMT3A and 3B) or DNA repair (APE1 and KU70) as well as with changes in the expression of genes involved in stress response, cell cycle control and bile acid biosynthesis. Other bacterial components analysed in this study did not lead to any significant changes in the tested molecular parameters. This study suggests that lipopolysaccharides are a major component of Gram-negative bacteria that induce molecular changes within naı¨ve cells of the host.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationKovalchuk, I., Walz, P., Thomas, J., & Kovalchuk, O. (2013). Genomic instability in liver cells caused by an LPS-induced bystander-like effecten_US
dc.identifier.urihttps://hdl.handle.net/10133/4638
dc.language.isoen_CAen_US
dc.publisherPublic Library of Scienceen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.subjectLiver cellsen_US
dc.subjectLipopolysaccharidesen_US
dc.subjectLPSen_US
dc.subjectGene expressionen_US
dc.subjectBacterial infectionen_US
dc.subjectGenome instabilityen_US
dc.subjectBystander effecten_US
dc.titleGenomic instability in liver cells caused by an LPS-induced bystander-like effecten_US
dc.typeArticleen_US
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