Expression analysis of FORKED-LIKE family genes during development and in response to NaCl, ABA, and auxin

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dc.contributor.authorClarke, Kurtis
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorSchultz, Elizabeth
dc.date.accessioned2018-07-27T15:22:54Z
dc.date.available2018-07-27T15:22:54Z
dc.date.issued2018
dc.degree.levelMastersen_US
dc.description.abstractAuxin based developmental processes rely on PINFORMED (PIN) proteins for the polarized export of auxin. The protein FORKED1 (FKD1) influences PIN1 polarity in provascular cells and the subsequent formation of closed loops in leaf venation. Mutations to FKD1 result in an open leaf vein pattern. FKD1 is a member of the 9-member FORKED-LIKE (FL) gene family. While single mutants of fl1, fl2, and fl3 result in no visible phenotype, a quadruple mutant of fkd1/fl1-2/ fl2/fl3 has severely disconnected veins and reduced root elongation. This study was designed to observe the expression patterns of FL-gene family members by production of promoter-reporter constructs and the modifications of their expression by hormones or abiotic stresses. I found that members of this gene family are regulated by auxin, supporting their role in vascular tissue development. Moreover, their response to abscisic acid may indicate that the family is regulated in response to abiotic stress.en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5175
dc.language.isoen_USen_US
dc.proquest.subject0307en_US
dc.proquest.subject0719en_US
dc.proquest.subject0479en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectPlant Physiologyen_US
dc.subjectFORKED-Likeen_US
dc.subjectLeaf Venationen_US
dc.subjectAbiotic Stressen_US
dc.subjectABAen_US
dc.subjectAuxinen_US
dc.subjectAbscisic aciden_US
dc.subjectArabidopsis thaliana -- Researchen_US
dc.subjectLeaf proteins -- Researchen_US
dc.subjectRoots (Botany) -- Effect of stress on -- Researchen_US
dc.subjectRoots (Botany) -- Morphogenesisen_US
dc.subjectMembrane proteinsen_US
dc.subjectPlants -- Effect of auxin on -- Researchen_US
dc.subjectPlants -- Effect of stress on -- Genetic aspects -- Researchen_US
dc.subjectLeaves --Effect of stress on -- Researchen_US
dc.subjectLeaves -- Morphogenesisen_US
dc.titleExpression analysis of FORKED-LIKE family genes during development and in response to NaCl, ABA, and auxinen_US
dc.typeThesisen_US
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