Comprehensive analysis of hepatitis B virus promoter region mutations

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dc.contributor.authorMeier-Stephenson, Vanessa
dc.contributor.authorBremner, William T. R.
dc.contributor.authorDalton, Chimone S.
dc.contributor.authorvan Marle, Guido
dc.contributor.authorCoffin, Carla S.
dc.contributor.authorPatel, Trushar R.
dc.date.accessioned2024-08-02T20:25:47Z
dc.date.available2024-08-02T20:25:47Z
dc.date.issued2018
dc.descriptionOpen access article. Creative Commons Attribution 4.0 International license (CC BY 4.0) applies
dc.description.abstractOver 250 million people are infected chronically with hepatitis B virus (HBV), the leading cause of liver cancer worldwide. HBV persists, due, in part, to its compact, stable minichromosome, the covalently-closed, circular DNA (cccDNA), which resides in the hepatocytes’ nuclei. Current therapies target downstream replication products, however, a true virological cure will require targeting the cccDNA. Finding targets on such a small, compact genome is challenging. For HBV, to remain replication-competent, it needs to maintain nucleotide fidelity in key regions, such as the promoter regions, to ensure that it can continue to utilize the necessary host proteins. HBVdb (HBV database) is a repository of HBV sequences spanning all genotypes (A–H) amplified from clinical samples, and hence implying an extensive collection of replication-competent viruses. Here, we analyzed the HBV sequences from HBVdb using bioinformatics tools to comprehensively assess the HBV core and X promoter regions amongst the nearly 70,000 HBV sequences for highly-conserved nucleotides and variant frequencies. Notably, there is a high degree of nucleotide conservation within specific segments of these promoter regions highlighting their importance in potential host protein-viral interactions and thus the virus’ viability. Such findings may have key implications for designing antivirals to target these areas.
dc.identifier.citationMeier-Stephenson, V., Bremner, W. T. R., Dalton, C. S., van Marle, G., Coffin, C. S., & Patel, T. R. (2018). Comprehensive analysis of hepatitis B virus promoter region mutations. Viruses, 10(11). Article 603. https://doi.org/10.3390/v10110603
dc.identifier.urihttps://hdl.handle.net/10133/6834
dc.language.isoen
dc.publisherMDPI
dc.publisher.departmentDepartment of Chemistry and Biochemistry
dc.publisher.facultyArts and Science
dc.publisher.institutionUniversity of Calgary
dc.publisher.institutionUniversity of Lethbridge
dc.publisher.institutionUniversity of Alberta
dc.subjectHepatitis B virus (HBV)
dc.subjectcccDNA
dc.subjectBasal core promoter
dc.subjectX promoter
dc.subjectSingle nucleotide polymorphisms
dc.subjectLogo analyses
dc.subjectGenotype alignments
dc.subjectPromoter regions
dc.subject.lcshHepatitis B virus
dc.titleComprehensive analysis of hepatitis B virus promoter region mutations
dc.typeArticle
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