Hydrogen-bonding interactions in T-2 toxin studies using solution and solid-state NMR

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dc.contributor.authorChaudhary, Praveen
dc.contributor.authorShank, Roxanne A.
dc.contributor.authorMontina, Tony
dc.contributor.authorGoettel, James T.
dc.contributor.authorForoud, Nora Afsaneh
dc.contributor.authorHazendonk, Paul
dc.contributor.authorEudes, François
dc.date.accessioned2016-07-05T21:08:13Z
dc.date.available2016-07-05T21:08:13Z
dc.date.issued2011
dc.descriptionOpen accessen_US
dc.description.abstractThe structure of T-2 toxin in the solid-state is limited to X-ray crystallographic studies, which lack sufficient resolution to provide direct evidence for hydrogen-bonding interactions. Furthermore, its solution-structure, despite extensive Nuclear Magnetic Resonance (NMR) studies, has provided little insight into its hydrogen-bonding behavior, thus far. Hydrogen-bonding interactions are often an important part of biological activity. In order to study these interactions, the structure of T-2 toxin was compared in both the solution- and solid-state using NMR Spectroscopy. It was determined that the solution- and solid-state structure differ dramatically, as indicated by differences in their carbon chemical shifts, these observations are further supported by solution proton spectral parameters and exchange behavior. The slow chemical exchange process and cross-relaxation dynamics with water observed between the hydroxyl hydrogen on C-3 and water supports the existence of a preferential hydrogen bonding interaction on the opposite side of the molecule from the epoxide ring, which is known to be essential for trichothecene toxicity. This result implies that these hydrogen-bonding interactions could play an important role in the biological function of T-2 toxin and posits towards a possible interaction for the trichothecene class of toxins and the ribosome. These findings clearly illustrate the importance of utilizing solid-state NMR for the study of biological compounds, and suggest that a more detailed study of this whole class of toxins, namely trichothecenes, should be pursued using this methodology.en_US
dc.description.peer-reviewYesen_US
dc.identifier.citationChaudhary, P., Shank, Roxanne A., Montina, T., Goettel, J.T., Foroud, N.A., Hazendonk, P., & Eudes, F. (2011). Hydrogen-bonding interactions in T-2 toxin studied using solution and solid-state NMR. Toxins, 3, 1310-1331. doi:10.3390/toxins3101310en_US
dc.identifier.urihttps://hdl.handle.net/10133/4554
dc.language.isoen_CAen_US
dc.publisherM D P I A Gen_US
dc.publisher.departmentDepartment of Chemistry & Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.publisher.institutionUniversity of Lethbridgeen_US
dc.publisher.institutionAgriculture and Agri-Food Canadaen_US
dc.subjectT-2 toxinen_US
dc.subjectTrichotheceneen_US
dc.subjectNMRen_US
dc.subjectHydrogen-bondingen_US
dc.subjectRibosomeen_US
dc.subjectToxinen_US
dc.subjectEpoxideen_US
dc.subjectWater bridgingen_US
dc.subjectDeuterium exchangeen_US
dc.subjectChemical exchangeen_US
dc.titleHydrogen-bonding interactions in T-2 toxin studies using solution and solid-state NMRen_US
dc.typeArticleen_US
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