Structural studies of RNA-protein complexes: a hybrid approach involving hydrodynamics, scattering, and computational methods

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dc.contributor.authorPatel, Trushar R.
dc.contributor.authorChojnowski, Grzegorz
dc.contributor.authorAstha
dc.contributor.authorKoul, Amit
dc.contributor.authorMcKenna, Sean A.
dc.contributor.authorBujnicki, Janusz M.
dc.date.accessioned2024-08-06T20:07:48Z
dc.date.available2024-08-06T20:07:48Z
dc.date.issued2017
dc.descriptionOpen access article. Creative Commons Attribution 4.0 Internatal license (CC BY 4.0) applies
dc.description.abstractThe diverse functional cellular roles played by ribonucleic acids (RNA) have emphasized the need to develop rapid and accurate methodologies to elucidate the relationship between the structure and function of RNA. Structural biology tools such as X-ray crystallography and Nuclear Magnetic Resonance are highly useful methods to obtain atomic-level resolution models of macromolecules. However, both methods have sample, time, and technical limitations that prevent their application to a number of macromolecules of interest. An emerging alternative to high-resolution structural techniques is to employ a hybrid approach that combines low-resolution shape information about macromolecules and their complexes from experimental hydrodynamic (e.g. analytical ultracentrifugation) and solution scattering measurements (e.g., solution X-ray or neutron scattering), with computational modeling to obtain atomic-level models. While promising, scattering methods rely on aggregation-free, monodispersed preparations and therefore the careful development of a quality control pipeline is fundamental to an unbiased and reliable structural determination. This review article describes hydrodynamic techniques that are highly valuable for homogeneity studies, scattering techniques useful to study the low-resolution shape, and strategies for computational modeling to obtain high-resolution 3D structural models of RNAs, proteins, and RNA-protein complexes.
dc.identifier.citationPatel, T. R., Chojnowski, G., Astha, Koul, A., McKenna, S. A., & Bujnicki, J. M. (2017). Structural studies of RNA-protein complexes: A hybrid approach involving hydrodynamics, scattering, and computational methods. Methods, 118-119, 146-162. https://doi.org/10.1016/j.ymeth.2016.12.002
dc.identifier.urihttps://hdl.handle.net/10133/6837
dc.language.isoen
dc.publisherElsevier
dc.publisher.institutionUniversity of Lethbridge
dc.publisher.institutionEuropean Molecular Biology Laboratory
dc.publisher.institutionInternational Institute of Molecular and Cell Biology in Warsaw
dc.publisher.institutionUniversity of Manitoba
dc.publisher.institutionAdam Mickiewicz University
dc.subjectAnalytical ultracentrifugation
dc.subjectComputational modeling
dc.subjectDisordered and flexible systems
dc.subjectDynamic light scattering
dc.subjectSize exclustion chromatography
dc.subjectSize exclusion chromatography coupled to multi-angle laser light scattering
dc.subjectSmall angle neutron scattering
dc.subjectSmall angle X-ray scattering
dc.subjectRNA-protein complexes
dc.subject.lcshRNA-protein interactions
dc.subject.lcshRNA
dc.subject.lcshProteins
dc.subject.lcshSmall-angle scattering
dc.subject.lcshGel permeation chromatography
dc.titleStructural studies of RNA-protein complexes: a hybrid approach involving hydrodynamics, scattering, and computational methods
dc.typeArticle
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