Cannabinol inhibits proliferation and induces cell cycle arrest and apoptosis in glioblastoma, hepatocellular carcinoma and breast cancer cells

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dc.contributor.authorZhong, Nuanying
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorKovalchuk, Igor
dc.date.accessioned2020-03-20T16:54:42Z
dc.date.available2020-03-20T16:54:42Z
dc.date.issued2020
dc.degree.levelMastersen_US
dc.description.abstractCannabis sativa is an agriculturally and medicinally important plant with many pharmaceutical properties. Cancer is a deadly disease; it is estimated that it will cause over 80 thousand deaths in 2019 in Canada. Although numerous studies have demonstrated that cannabinoids have anti-tumorous properties in various cancers, the anti-malignant activities of cannabinol (CBN) on carcinogenesis and underlying mechanisms remain largely unknown. In this study, we provide evidence that CBN inhibits proliferation of A172, HB8065 and HCC1806 cells in a dose- and time-dependent manner. CBN regulates expression of cannabinoid receptors, CB2, GPR55 and GPR18 in different cell lines, while reducing levels of phosphorylated ERK1/2 in HCC1806 and phosphorylated AKT in A172 and HB8065 cells. We find that CBN induces apoptosis through downregulation of p21 and p27 and a G1 or S-phase cell cycle arrest through a dose-dependent downregulation of cyclin E1, CDK1 and CDK2. These data support the medicinal potential of CBN in anti-cancer therapy.en_US
dc.description.sponsorshipMitacsen_US
dc.identifier.urihttps://hdl.handle.net/10133/5697
dc.language.isoen_USen_US
dc.proquest.subjectCellular biology [0379]en_US
dc.proquest.subjectOncology [0992]en_US
dc.proquest.subjectMolecular biology [0307]en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciencesen_US
dc.publisher.departmentDepartment of Biological Sciencesen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subjectCancer cellsen_US
dc.subjectCannabinoidsen_US
dc.subjectCannabis — Therapeutic useen_US
dc.subjectCarcinogenesisen_US
dc.subjectMarijuana — Therapeutic useen_US
dc.subjectMedical Marijuana — therapeutic useen_US
dc.titleCannabinol inhibits proliferation and induces cell cycle arrest and apoptosis in glioblastoma, hepatocellular carcinoma and breast cancer cellsen_US
dc.typeThesisen_US
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