Development of RNA-based translation modulation tools for synthetic biology applications

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dc.contributor.authorHakstol, Rhys J.
dc.contributor.authorUniversity of Lethbridge. Faculty of Arts and Science
dc.contributor.supervisorWieden, Hans-Joachim
dc.date.accessioned2019-07-10T20:22:36Z
dc.date.available2019-07-10T20:22:36Z
dc.date.issued2019
dc.degree.levelMastersen_US
dc.description.abstractThe ability to reliably regulate translation of heterologous proteins is of great interest for diverse applications in the field of biological and metabolic engineering. RNA-based tools to control ribosome-dependent synthesis of proteins within E. coli were developed. First, adaptation of the well-studied RNA-IN and RNA-OUT system based on E. coli Tn10 to reliably repress translation regardless of coding sequence context was performed. Using an invariant sequence within the RNA-IN/OUT interacting region does not interfere with the function of the RNAs involved. The same RNA-IN/RNA-OUT system was adapted to activate the translation of a specific target mRNA. Regulation of translation extends to processing of polyproteins. In eukaryotes this can be accomplished by viral 2A peptides, avoiding reinitiating at a downstream start codon but has not been observed in bacteria. Therefore, a strategy for developing libraries of 2A peptides with potential activity across an array of organisms was developed.en_US
dc.embargoNoen_US
dc.identifier.urihttps://hdl.handle.net/10133/5456
dc.language.isoen_USen_US
dc.proquest.subject0379en_US
dc.proquest.subject0307en_US
dc.proquest.subject0410en_US
dc.proquestyesYesen_US
dc.publisherLethbridge, Alta. : Universtiy of Lethbridge, Department of Mathematics and Computer Scienceen_US
dc.publisher.departmentDepartment of Chemistry and Biochemistryen_US
dc.publisher.facultyArts and Scienceen_US
dc.relation.ispartofseriesThesis (University of Lethbridge. Faculty of Arts and Science)en_US
dc.subject2A-peptidesen_US
dc.subjectbiochemistryen_US
dc.subjectgene expression regulationen_US
dc.subjectnovel trans-acting RNAsen_US
dc.subjectprokaryotic gene expressionen_US
dc.subjectsynthetic biologyen_US
dc.subjectDissertations, Academicen_US
dc.titleDevelopment of RNA-based translation modulation tools for synthetic biology applicationsen_US
dc.typeThesisen_US
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